LOCUS       sequence-31372-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-HSP70 (3L6Q)
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     273..292
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     376..395
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        376..394
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    395..419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             434..456
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             466..483
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             496..516
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctgattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
       61 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
      121 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
      181 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
      241 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
      301 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
      361 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
      421 ctctagaaat aattttgttt aactttaaga aggagatata ccatgcatca tcatcatcat
      481 cacagcagcg gcagagaaaa cttgtatttc cagggctcat ctgaaggtcc agctattggt
      541 attgatttag gtaccacata ctcatgtgtt ggtgtatgga gaaacgatac tgtagatatt
      601 gttccaaacg atcaaggtaa ccgtacaaca ccatcatatg tagcatttac agaaactgag
      661 cgtcttattg gtgatgcagc aaagaaccaa gttgctagaa acccagaaaa tacagttttt
      721 gatgctaaac gtcttattgg tagaaagttt gatgatcagg cagtacaaag tgatatgact
      781 cactggccat ttaaagtagt tagaggtcca aaggacaagc caatcatcag tgtaaactac
//
