LOCUS       sequence-31373-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-PYRK1 (3MA8)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             437..448
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgcatca tcatcatcat cacagcagcg
       61 gcagagaaaa cttgtatttc cagggcgcat ctacttctgc agtaatgagt tgcacactgg
      121 gaaaggcaac ttgtttaggg atggacaaaa tttgctctcc tttagcagat aatgacgtaa
      181 cccaaaggaa gacacaaatc atatgtacaa ttggcccaag ctgtaataat gtagaatcgt
      241 tgattggttt aattgacaaa ggtatgagtg ttgctagact taacttttcc catggcgatc
      301 atgagagtca cttcaagact ttacaaaata tcagggaggc agctaaagca agacctcact
      361 ctaccgttgg gataatgcta gatactaaag gcccagagat cagaaccgga atgttggaag
      421 gtggaaagcc cattgaattg aaggcaggcc aaactttaaa aattacaaca gattactcta
      481 tgttaggtaa ctccgaatgt atatcgtgta gctattctct attacctaaa agtgtacaaa
      541 ttggaagtac tgttttaatt gctgatggct cgttatcgac tcaagtgcta gaaattggcg
      601 atgatttcat tgtctgcaag gtattgaata gcgtaactat cggtgaaagg aaaaatatga
      661 atctaccagg ttgtaaggtc cacttaccaa taattgggga taaagataga catgacattg
      721 tggactttgc ccttaagtac aacttggatt tttattgctc tttccttcgt gcagaatgga
      781 gctgatgtgc aactatgta
//
