LOCUS       sequence-313736-        8871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, 
            Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP
  TITLE     Dual DNA and protein tagging of open chromatin unveils dynamics of 
            epigenomic landscapes in leukemia.
  JOURNAL   Nat Methods. 2021 Mar;18(3):293-302. doi: 
            10.1038/s41592-021-01077-8. Epub 2021 Mar 1.
  PUBMED    33649590
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01077-8"; journalName: "Nat Methods"; date: 
            "2021-03"; volume: "18"; issue: "3"; pages: "293-302"
COMMENT     Sequence Label: pTXB1-TP5
FEATURES             Location/Qualifiers
     source          1..8871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      231..317
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      414..500
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      597..683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      780..866
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      963..1049
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1184..1203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1184..1202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1203..1227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1271..2698
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             2717..3463
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             3464..3487
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3488..4081
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             4112..4267
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(4332..4350)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4346..4393
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4453..4557
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4558..4626,4627..5418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4776..4795)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5460..5973)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5655..5676)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5867..5886
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6084..6672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6573..6592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6826..6843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6865..6884)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7025..7047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7041..7232)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7789..8871)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8833..8852
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgcc
      181 ggcatgccgc cctttcgtct tcaagaatta attcccaatt ccccaggcat caaataaaac
      241 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      301 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
      361 ggtggcgggc aggacgcccg ccataaactg ccaggaatta attccccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      541 gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc aggcatcaaa
      601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      661 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      721 ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc cccaggcatc
      781 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      841 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
      901 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa ttccccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     1081 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat tggggatcgg
     1141 aattaattcc cggtttaaac cggggatctc gatcccgcga aattaatacg actcactata
     1201 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgttaac tttaagaagg
     1261 agatatacat atgattacca gtgcactgca tcgtgcggcg gattgggcga aaagcgtgtt
     1321 ttctagtgct gcgctgggtg atccgcgtcg taccgcgcgt ctggtgaatg ttgcggcgca
     1381 actggccaaa tatagcggca aaagcattac cattagcagc gaaggcagca aagccatgca
     1441 ggaaggcgcg tatcgtttta ttcgtaatcc gaacgtgagc gcggaagcga ttcgtaaagc
     1501 gggtgccatg cagaccgtga aactggccca ggaatttccg gaactgctgg caattgaaga
     1561 taccacctct ctgagctatc gtcatcaggt ggcggaagaa ctgggcaaac tgggtagcat
     1621 tcaggataaa agccgtggtt ggtgggtgca tagcgtgctg ctgctggaag cgaccacctt
     1681 tcgtaccgtg ggcctgctgc atcaagaatg gtggatgcgt ccggatgatc cggcggatgc
     1741 ggatgaaaaa gaaagcggca aatggctggc cgctgctgca acttcgcgtc tgagaatggg
     1801 cagcatgatg agcaacgtga ttgcggtgtg cgatcgtgaa gcggatattc atgcgtatct
     1861 gcaagataaa ctggcccata acgaacgttt tgtggtgcgt agcaaacatc cgcgtaaaga
     1921 tgtggaaagc ggcctgtatc tgtatgatca cctgaaaaac cagccggaac tgggcggcta
     1981 tcagattagc attccgcaga aaggcgtggt ggataaacgt ggcaaacgta aaaaccgtcc
     2041 ggcgcgtaaa gcgagcctga gcctgcgtag cggccgtatt accctgaaac agggcaacat
     2101 taccctgaac gcggtgctgg ccgaagaaat taatccgccg aaaggcgaaa ccccgctgaa
     2161 atggctgctg ctgaccagcg agccggtgga aagtctggcc caagcgctgc gtgtgattga
     2221 tatttatacc catcgttggc gcattgaaga atttcacaaa gcgtggaaaa cgggtgcggg
     2281 tgcggaacgt cagcgtatgg aagaaccgga taacctggaa cgtatggtga gcattctgag
     2341 ctttgtggcg gtgcgtctgc tgcaactgcg tgaatctttt actccgccgc aagcactgcg
     2401 tgcgcagggc ctgctgaaag aagcggaaca cgttgaaagc cagagcgcgg aaaccgtgct
     2461 gaccccggat gaatgccaac tgctgggcta tctggataaa ggcaaacgca aacgcaaaga
     2521 aaaagcgggc agcctgcaat gggcgtatat ggcgattgcg cgtctgggcg gctttatgga
     2581 tagcaaacgt accggcattg cgagctgggg tgcgctgtgg gaaggttggg aagcgctgca
     2641 aagcaaactg gatggctttc tggccgcgaa agacctgatg gcgcagggca ttaaaatcgg
     2701 atccggtgca ggcgccggaa agtcttaccc aactgtgagt gctgattacc aggacgccgt
     2761 tgagaaggcg aagaagaagc tcagaggctt catcgctgag aagagatgcg ctcctctaat
     2821 gctccgtttg gcattccact ctgctggaac ctttgacaag ggcacgaaga ccggtggacc
     2881 cttcggaacc atcaagcacc ctgccgaact ggctcacagc gctaacaacg gtcttgacat
     2941 cgctgttagg cttttggagc cactcaaggc ggagttccct attttgagct acgccgattt
     3001 ctaccagttg gctggcgttg ttgccgttga ggtcacgggt ggacctaagg ttccattcca
     3061 ccctggaaga gaggacaagc ctgagccacc accagagggt cgcttgcccg atcccactaa
     3121 gggttctgac catttgagag atgtgtttgg caaagctatg gggcttactg accaagatat
     3181 cgttgctcta tctgggggtc acactattgg agctgcacac aaggagcgtt ctggatttga
     3241 gggtccctgg acctctaatc ctcttatttt cgacaactca tacttcacgg agttgttgag
     3301 tggtgagaag gaaggtctcc ttcagctacc ttctgacaag gctcttttgt ctgaccctgt
     3361 attccgccct ctcgttgaca aatatgcagc ggacgaagat gccttctttg ctgattacgc
     3421 tgaggctcac caaaagcttt ccgagcttgg gtttgctgat gccgattaca aggatgacga
     3481 cgataagtgc atcacgggag atgcactagt tgccctaccc gagggcgagt cggtacgcat
     3541 cgccgacatc gtgccgggtg cgcggcccaa cagtgacaac gccatcgacc tgaaagtcct
     3601 tgaccggcat ggcaatcccg tgctcgccga ccggctgttc cactccggcg agcatccggt
     3661 gtacacggtg cgtacggtcg aaggtctgcg tgtgacgggc accgcgaacc acccgttgtt
     3721 gtgtttggtc gacgtcgccg gggtgccgac cctgctgtgg aagctgatcg acgaaatcaa
     3781 gccgggcgat tacgcggtga ttcaacgcag cgcattcagc gtcgactgtg caggttttgc
     3841 ccgcgggaaa cccgaatttg cgcccacaac ctacacagtc ggcgtccctg gactggtgcg
     3901 tttcttggaa gcacaccacc gagacccgga cgcccaagct atcgccgacg agctgaccga
     3961 cgggcggttc tactacgcga aagtcgccag tgtcaccgac gccggcgtgc agccggtgta
     4021 tagccttcgt gtcgacacgg cagaccacgc gtttatcacg aacgggttcg tcagccacgc
     4081 tactggcctc accggtctga actcaggcct cacgacaaat cctggtgtat ccgcttggca
     4141 ggtcaacaca gcttatactg cgggacaatt ggtcacatat aacggcaaga cgtataaatg
     4201 tttgcagccc cacacctcct tggcaggatg ggaaccatcc aacgttcctg ccttgtggca
     4261 gcttcaatga ctgcaggaag gggatccggc tgctaacaaa gcccgaaagg aagctgagtt
     4321 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     4381 gaggggtttt ttgctgaaag gaggaactat atccggataa ctacgtcagg tggcactttt
     4441 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4501 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4561 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4621 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4681 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4741 gaacgtttcc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4801 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4861 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4921 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4981 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5041 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5101 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5161 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5221 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5281 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5341 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5401 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5461 ccccggttga taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa
     5521 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5581 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag
     5641 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5701 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat
     5761 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5821 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5881 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5941 ccgccgcgct taatgcgccg ctatagggcg cgtaaaagga tctaggtgaa gatccttttt
     6001 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6841 gaggaagcta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     6901 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     6961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     7081 ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc
     7141 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     7201 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg
     7261 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     7321 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     7381 cgggaccaga gaaaaatcac tcagggtcaa tgccagccga acgccagcaa gacgtagccc
     7441 agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg
     7501 ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg
     7561 ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc
     7621 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc
     7681 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
     7741 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
     7801 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7861 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     7921 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     7981 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     8041 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
     8101 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     8161 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     8221 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     8281 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     8341 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     8401 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     8461 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     8521 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     8581 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     8641 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     8701 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     8761 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     8821 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca c
//
