LOCUS       sequence-313737-        8868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, 
            Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP
  TITLE     Dual DNA and protein tagging of open chromatin unveils dynamics of 
            epigenomic landscapes in leukemia.
  JOURNAL   Nat Methods. 2021 Mar;18(3):293-302. doi: 
            10.1038/s41592-021-01077-8. Epub 2021 Mar 1.
  PUBMED    33649590
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-021-01077-8"; journalName: "Nat Methods"; date: 
            "2021-03"; volume: "18"; issue: "3"; pages: "293-302"
COMMENT     Sequence Label: pTXB1-TP2
FEATURES             Location/Qualifiers
     source          1..8868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      231..317
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      414..500
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      597..683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      780..866
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      963..1049
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1184..1203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1184..1202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1203..1227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1271..2698
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="MITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKS
                     ITISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYR
                     HQVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESG
                     KWLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGL
                     YLYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNA
                     VLAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAER
                     QRMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTP
                     DECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQS
                     KLDGFLAAKDLMAQGIKI"
     CDS             2714..3460
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             3461..3484
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3485..4078
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             4109..4264
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(4329..4347)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4343..4390
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4450..4554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4555..4623,4624..5415)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4773..4792)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5457..5970)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5652..5673)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5864..5883
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6081..6669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6570..6589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6823..6840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6862..6881)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7022..7044
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7038..7229)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7786..8868)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8830..8849
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgcc
      181 ggcatgccgc cctttcgtct tcaagaatta attcccaatt ccccaggcat caaataaaac
      241 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      301 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
      361 ggtggcgggc aggacgcccg ccataaactg ccaggaatta attccccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
      541 gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc aggcatcaaa
      601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      661 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      721 ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc cccaggcatc
      781 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      841 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
      901 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa ttccccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     1081 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat tggggatcgg
     1141 aattaattcc cggtttaaac cggggatctc gatcccgcga aattaatacg actcactata
     1201 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgttaac tttaagaagg
     1261 agatatacat atgattacca gtgcactgca tcgtgcggcg gattgggcga aaagcgtgtt
     1321 ttctagtgct gcgctgggtg atccgcgtcg taccgcgcgt ctggtgaatg ttgcggcgca
     1381 actggccaaa tatagcggca aaagcattac cattagcagc gaaggcagca aagccatgca
     1441 ggaaggcgcg tatcgtttta ttcgtaatcc gaacgtgagc gcggaagcga ttcgtaaagc
     1501 gggtgccatg cagaccgtga aactggccca ggaatttccg gaactgctgg caattgaaga
     1561 taccacctct ctgagctatc gtcatcaggt ggcggaagaa ctgggcaaac tgggtagcat
     1621 tcaggataaa agccgtggtt ggtgggtgca tagcgtgctg ctgctggaag cgaccacctt
     1681 tcgtaccgtg ggcctgctgc atcaagaatg gtggatgcgt ccggatgatc cggcggatgc
     1741 ggatgaaaaa gaaagcggca aatggctggc cgctgctgca acttcgcgtc tgagaatggg
     1801 cagcatgatg agcaacgtga ttgcggtgtg cgatcgtgaa gcggatattc atgcgtatct
     1861 gcaagataaa ctggcccata acgaacgttt tgtggtgcgt agcaaacatc cgcgtaaaga
     1921 tgtggaaagc ggcctgtatc tgtatgatca cctgaaaaac cagccggaac tgggcggcta
     1981 tcagattagc attccgcaga aaggcgtggt ggataaacgt ggcaaacgta aaaaccgtcc
     2041 ggcgcgtaaa gcgagcctga gcctgcgtag cggccgtatt accctgaaac agggcaacat
     2101 taccctgaac gcggtgctgg ccgaagaaat taatccgccg aaaggcgaaa ccccgctgaa
     2161 atggctgctg ctgaccagcg agccggtgga aagtctggcc caagcgctgc gtgtgattga
     2221 tatttatacc catcgttggc gcattgaaga atttcacaaa gcgtggaaaa cgggtgcggg
     2281 tgcggaacgt cagcgtatgg aagaaccgga taacctggaa cgtatggtga gcattctgag
     2341 ctttgtggcg gtgcgtctgc tgcaactgcg tgaatctttt actccgccgc aagcactgcg
     2401 tgcgcagggc ctgctgaaag aagcggaaca cgttgaaagc cagagcgcgg aaaccgtgct
     2461 gaccccggat gaatgccaac tgctgggcta tctggataaa ggcaaacgca aacgcaaaga
     2521 aaaagcgggc agcctgcaat gggcgtatat ggcgattgcg cgtctgggcg gctttatgga
     2581 tagcaaacgt accggcattg cgagctgggg tgcgctgtgg gaaggttggg aagcgctgca
     2641 aagcaaactg gatggctttc tggccgcgaa agacctgatg gcgcagggca ttaaaatccc
     2701 agctccagct ccaggaaagt cttacccaac tgtgagtgct gattaccagg acgccgttga
     2761 gaaggcgaag aagaagctca gaggcttcat cgctgagaag agatgcgctc ctctaatgct
     2821 ccgtttggca ttccactctg ctggaacctt tgacaagggc acgaagaccg gtggaccctt
     2881 cggaaccatc aagcaccctg ccgaactggc tcacagcgct aacaacggtc ttgacatcgc
     2941 tgttaggctt ttggagccac tcaaggcgga gttccctatt ttgagctacg ccgatttcta
     3001 ccagttggct ggcgttgttg ccgttgaggt cacgggtgga cctaaggttc cattccaccc
     3061 tggaagagag gacaagcctg agccaccacc agagggtcgc ttgcccgatc ccactaaggg
     3121 ttctgaccat ttgagagatg tgtttggcaa agctatgggg cttactgacc aagatatcgt
     3181 tgctctatct gggggtcaca ctattggagc tgcacacaag gagcgttctg gatttgaggg
     3241 tccctggacc tctaatcctc ttattttcga caactcatac ttcacggagt tgttgagtgg
     3301 tgagaaggaa ggtctccttc agctaccttc tgacaaggct cttttgtctg accctgtatt
     3361 ccgccctctc gttgacaaat atgcagcgga cgaagatgcc ttctttgctg attacgctga
     3421 ggctcaccaa aagctttccg agcttgggtt tgctgatgcc gattacaagg atgacgacga
     3481 taagtgcatc acgggagatg cactagttgc cctacccgag ggcgagtcgg tacgcatcgc
     3541 cgacatcgtg ccgggtgcgc ggcccaacag tgacaacgcc atcgacctga aagtccttga
     3601 ccggcatggc aatcccgtgc tcgccgaccg gctgttccac tccggcgagc atccggtgta
     3661 cacggtgcgt acggtcgaag gtctgcgtgt gacgggcacc gcgaaccacc cgttgttgtg
     3721 tttggtcgac gtcgccgggg tgccgaccct gctgtggaag ctgatcgacg aaatcaagcc
     3781 gggcgattac gcggtgattc aacgcagcgc attcagcgtc gactgtgcag gttttgcccg
     3841 cgggaaaccc gaatttgcgc ccacaaccta cacagtcggc gtccctggac tggtgcgttt
     3901 cttggaagca caccaccgag acccggacgc ccaagctatc gccgacgagc tgaccgacgg
     3961 gcggttctac tacgcgaaag tcgccagtgt caccgacgcc ggcgtgcagc cggtgtatag
     4021 ccttcgtgtc gacacggcag accacgcgtt tatcacgaac gggttcgtca gccacgctac
     4081 tggcctcacc ggtctgaact caggcctcac gacaaatcct ggtgtatccg cttggcaggt
     4141 caacacagct tatactgcgg gacaattggt cacatataac ggcaagacgt ataaatgttt
     4201 gcagccccac acctccttgg caggatggga accatccaac gttcctgcct tgtggcagct
     4261 tcaatgactg caggaagggg atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     4321 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     4381 gggttttttg ctgaaaggag gaactatatc cggataacta cgtcaggtgg cacttttcgg
     4441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4741 cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     4801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaccc
     5461 cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg
     5521 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     5581 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagggt
     5641 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     5701 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa
     5761 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     5821 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     5881 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     5941 ccgcgcttaa tgcgccgcta tagggcgcgt aaaaggatct aggtgaagat cctttttgat
     6001 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6841 gaagctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     6901 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     6961 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7021 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     7081 cggtaaagct catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg
     7141 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     7201 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     7261 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     7321 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     7381 gaccagagaa aaatcactca gggtcaatgc cagccgaacg ccagcaagac gtagcccagc
     7441 gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
     7501 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     7561 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     7621 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg
     7681 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     7741 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     7801 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7861 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     7921 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     7981 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     8041 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     8101 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     8161 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     8221 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     8281 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     8341 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     8401 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     8461 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     8521 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     8581 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     8641 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     8701 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     8761 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     8821 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcac
//
