LOCUS       sequence-313738-        3747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Hanish JP, Yanowitz JL, de Lange T
  TITLE     Stringent sequence requirements for the formation of human 
            telomeres.
  JOURNAL   Proc Natl Acad Sci U S A. 1994 Sep 13. 91(19):8861-5.
  PUBMED    8090736
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1994 Sep 13. 91(19):8861-5."
COMMENT     Sequence Label: pSXneo 270(T2AG3)
FEATURES             Location/Qualifiers
     source          1..3747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    71..119
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(126..929)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(237..256)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     847..866
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1402..1420)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1403..1420)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1501..1520)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1620..1642
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1680..1698)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1766..1870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1871..1939,1940..2731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2089..2108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2902..3490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3391..3410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3644..3661
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatacgact cactataggg agaccggcct cgacctgcag gcatgcaagc ttcagctgct
       61 cgacggatcc gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccga
      121 tcccctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
      181 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
      241 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
      301 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
      361 gtcacgacga gatcatcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
      421 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
      481 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
      541 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
      601 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
      661 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
      721 agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
      781 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
      841 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
      901 aatccatctt gttcaatggc cgatcccata ttggctgcag ggtcgctcgg tgttcgaggc
      961 cacacgcgtc accttaatat gcgaagtgga cctgggaccg cgccgccccg actgcatctg
     1021 cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgctcgacat tgggtggaaa
     1081 cattccaggc ctgggtggag aggctttttg cttcctcttg caaaaccaca ctgctcgaca
     1141 ttgggtggaa acattccagg cctgggtgga gaggcttttt gcttcctctt gaaaaccaca
     1201 ctgctcgact ctagaggatc cccgggtacc gagctcgaat tccgcggaat tccggttggg
     1261 gttggggttg gggttggggt tggggttagg gttggggtta gggttagggt tagggttagg
     1321 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tcggaattgc
     1381 ggccgcatcg atagatctgg ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     1441 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tccatatggt gcactctcag
     1501 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     1561 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     1621 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     1681 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     1741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     1801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     1861 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     1921 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1981 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     2041 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     2101 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     2161 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     2221 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     2281 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     2341 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     2401 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     2461 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     2521 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     2581 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2641 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2701 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     2761 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2821 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3541 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3601 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3661 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3721 atgcaggtta acctggctta tcgaaat
//
