LOCUS       sequence-31375-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-LAMMER
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     274..293
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    396..420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             435..457
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             467..484
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             497..517
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cctgattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
       61 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
      121 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
      181 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
      241 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
      301 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      361 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
      421 cctctagaaa taattttgtt taactttaag aaggagatat accatgcatc atcatcatca
      481 tcacagcagc ggcagagaaa acttgtattt ccagggcgat gatgaaattg tccattttag
      541 ttggaagaaa ggtatgctat taaataatgc ctttttagtt ataagaaaaa tgggagatgg
      601 gacatttggt agagttttat tatgtcaaca tatagataat aaaaaatact acgctgtaaa
      661 agttgttcga aacataaaga aatatacacg ctcagctaaa attgaagcag atattttaaa
      721 aaaaatacaa aatgatgata ttaataataa taatattgtt aagtatcatg ggaaatttat
      781 gtattatgac catatgtgtt taatatttga accattaggt ccatcattat atgaaataat
      841 tacaaggaat aattataacg gattccatat agaa
//
