LOCUS       sequence-313762-        7905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD33 vector encoding MotA, eGFP-MotB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F
  TITLE     Impact of fluorescent protein fusions on the bacterial flagellar 
            motor.
  JOURNAL   Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w.
  PUBMED    28974721
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-10-3"; volume: "7(1):12583. doi"; pages: " 
            10.1038/s41598-017-11241-w"
COMMENT     Sequence Label: pMH-02
FEATURES             Location/Qualifiers
     source          1..7905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..293
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     216..235
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             join(1234..1236,1237..1239,1240..1950)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1234..1236,1237..1239,1240..1950)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1279..1300)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1540..1559)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1887..1908
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2947..2964)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3117..3203
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3295..3322
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3341..3432
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3890..4345
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3977..3996)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4187..4208
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4901..5560)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5475..5494
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5561..5663)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6055..6072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6189..6734)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6269..6288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6810..6829
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(7009..7887)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
       61 ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
      121 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
      181 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
      241 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
      301 tttttgggct agcgaattcg agctcggtac ccgaaggatg atgtcgtgct tatcttatta
      361 ggttacctgg ttgttctcgg tacagttttc ggcggttatt tgatgaccgg tggaagcctt
      421 ggagcactct atcaacccgc tgaactggtg attattgccg gtgcagggat tgggtcgttt
      481 atcgtcggca ataatggcaa agcgattaaa ggcacgctga aggcgctgcc gttgctgttt
      541 cgtcgctcca aatacaccaa agcaatgtat atggatctgc tggctctgct ttatcggttg
      601 atggcgaaat cgcggcagat ggggatgttt tcgctggaac gtgatattga aaatccccgt
      661 gagagcgaga tcttcgccag ctacccacgc atcctcgcgg atagcgtcat gcttgatttt
      721 atcgtcgatt atctgcgcct gattatcagc ggtcacatga acaccttcga aatcgaagct
      781 ctgatggatg aagagattga gacgcacgaa agcgaggcag aagtcccggc gaacagtctg
      841 gcgctggtcg gggactcact tccggcgttt ggtattgttg cggctgtaat gggggtcgtt
      901 cacgcgttag gttcagccga tcgtcctgcc gccgagctgg gtgcgcttat cgcacatgcg
      961 atggtgggga ctttcctcgg cattttattg gcttacggat ttatttcccc attagcgact
     1021 gttttacgtc agaaaagcgc cgaaaccagc aaaatgatgc agtgcgtcaa agtcactctg
     1081 ctttctaatc tgaacggtta cgcaccgcct atcgccgttg agtttggtcg caaaacgctc
     1141 tattccagcg aacgtccgtc gtttattgaa ctggaagagc atgtgcgtgc ggtgaaaaat
     1201 ccgcaacaac agacgacaac cgaggaagca tgaatggtga gcaagggcga ggagctgttc
     1261 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1321 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1381 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1441 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1501 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1561 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1621 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1681 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1741 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1921 atcactctcg gcatggacga gctgtacaag atgaagaatc aagcgcatcc gattattgtc
     1981 gtcaaacgac gcaaagccaa aagccacggg gcagcacatg gatcgtggaa gattgcttat
     2041 gccgacttta tgactgcgat gatggccttt tttctggtga tgtggctgat ctccatctcc
     2101 agcccaaaag agctgattca gattgcggag tacttccgga ctccactggc gactgcggtt
     2161 acgggcggcg atcgcatttc taatagtgaa agcccaattc ccggcggtgg tgatgattac
     2221 acccaaagcc agggggaagt gaataagcag ccgaacatcg aagagctgaa aaaacgcatg
     2281 gagcaaagtc gattgcggaa attgcggggt gatctcgacc agttgataga gtccgatccg
     2341 aaactgcggg cgttacgtcc ccatctcaaa atcgatctgg tccaggaagg tctacgtatt
     2401 cagatcatcg atagccagaa tcgcccgatg tttagaaccg gcagtgccga tgtcgaaccc
     2461 tatatgcgcg acattctgcg cgccattgcg cctgtactga acggtattcc caaccgtatt
     2521 agcctttcag gtcataccga tgatttcccc tacgccagcg gtgagaaagg atatagcaac
     2581 tgggagcttt ctgccgatcg ggccaatgca tcccgccgcg aactgatggt cggagggttg
     2641 gatagcggca aagtgttacg tgtcgtcggc atggcggcaa cgatgcgctt aagcgatcgc
     2701 ggacctgatg atgccgtcaa ccgtcgcatc agcctgctgg tactgaacaa acaagccgaa
     2761 caggccattt tgcatgaaaa cgccgaaagc cagaatgagc cagtaagcgc cctggaaaaa
     2821 cctgaggttg caccacaggt cagtgttccc acaatgccat cagccgaacc gaggtgaggg
     2881 gatcctctag gtcgacctgc aggcatgcaa gcttggctgt tttggcggat gagagaagat
     2941 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
     3001 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
     3061 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
     3121 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     3181 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     3241 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     3301 ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata
     3361 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3421 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3481 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3541 cagttgggtg cagcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3601 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3661 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3721 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3781 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3841 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta cgcgccctgt
     3901 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3961 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4021 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4081 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4141 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4201 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     4261 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4321 aacaaaatat taacgtttac aatttaaaag gatctaggtg aagatccttt ttgataatct
     4381 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4561 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4681 gttaccagtc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
     4741 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
     4801 tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     4861 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     4921 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     4981 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     5041 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     5101 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     5161 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     5221 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     5281 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     5341 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     5401 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     5461 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     5521 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     5581 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     5641 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     5701 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     5761 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg
     5821 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
     5881 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac
     5941 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     6001 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     6061 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     6121 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     6181 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     6241 cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggcggctcc
     6301 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     6361 cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
     6421 ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta actatcgtct
     6481 tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg gtaattgatt
     6541 tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg acaagttttg
     6601 gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct
     6661 tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt acgcgcagac
     6721 caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag gctcatgagc
     6781 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     6841 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ctgctcatgt
     6901 ttgacagctt atcatcgatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca
     6961 aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt
     7021 gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc
     7081 ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc
     7141 gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg
     7201 gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg
     7261 cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag
     7321 gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga
     7381 ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag
     7441 ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa
     7501 atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca
     7561 gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc
     7621 gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc
     7681 acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt
     7741 gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc
     7801 gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag
     7861 caggggatca ttttgcgctt cagccatact tttcatactc ccgcc
//
