LOCUS       sequence-313770-        7878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD33 vector encoding MotA, yPET-MotB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F
  TITLE     Impact of fluorescent protein fusions on the bacterial flagellar 
            motor.
  JOURNAL   Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w.
  PUBMED    28974721
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-10-3"; volume: "7(1):12583. doi"; pages: " 
            10.1038/s41598-017-11241-w"
COMMENT     Sequence Label: pMH-03
FEATURES             Location/Qualifiers
     source          1..7878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..287
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     210..229
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1228..1941
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     primer_bind     complement(2914..2931)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3084..3170
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3262..3289
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3308..3399
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3857..4312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3944..3963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4154..4175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4868..5527)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5442..5461
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5528..5630)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6022..6039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6156..6701)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6236..6255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6777..6796
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(6976..7854)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct
       61 cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac
      121 caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat
      181 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc
      241 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg
      301 ggctagcgaa ttcgagctcg gtacccgaag gatgatgtcg tgcttatctt attaggttac
      361 ctggttgttc tcggtacagt tttcggcggt tatttgatga ccggtggaag ccttggagca
      421 ctctatcaac ccgctgaact ggtgattatt gccggtgcag ggattgggtc gtttatcgtc
      481 ggcaataatg gcaaagcgat taaaggcacg ctgaaggcgc tgccgttgct gtttcgtcgc
      541 tccaaataca ccaaagcaat gtatatggat ctgctggctc tgctttatcg gttgatggcg
      601 aaatcgcggc agatggggat gttttcgctg gaacgtgata ttgaaaatcc ccgtgagagc
      661 gagatcttcg ccagctaccc acgcatcctc gcggatagcg tcatgcttga ttttatcgtc
      721 gattatctgc gcctgattat cagcggtcac atgaacacct tcgaaatcga agctctgatg
      781 gatgaagaga ttgagacgca cgaaagcgag gcagaagtcc cggcgaacag tctggcgctg
      841 gtcggggact cacttccggc gtttggtatt gttgcggctg taatgggggt cgttcacgcg
      901 ttaggttcag ccgatcgtcc tgccgccgag ctgggtgcgc ttatcgcaca tgcgatggtg
      961 gggactttcc tcggcatttt attggcttac ggatttattt ccccattagc gactgtttta
     1021 cgtcagaaaa gcgccgaaac cagcaaaatg atgcagtgcg tcaaagtcac tctgctttct
     1081 aatctgaacg gttacgcacc gcctatcgcc gttgagtttg gtcgcaaaac gctctattcc
     1141 agcgaacgtc cgtcgtttat tgaactggaa gagcatgtgc gtgcggtgaa aaatccgcaa
     1201 caacagacga caaccgagga agcatgaatg tctaaaggtg aagaattatt cactggtgtt
     1261 gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt
     1321 gaaggtgaag gtgatgctac gtacggtaaa ttgaccttaa aattactctg tactactggt
     1381 aaattgccag ttccatggcc aaccttagtc actactttag gttatggtgt tcaatgtttt
     1441 gctagatacc cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt
     1501 tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa
     1561 gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa
     1621 gaagatggta acattttagg tcacaaattg gaatacaact ataactctca caatgtttac
     1681 atcactgctg acaaacaaaa gaatggtatc aaagctaact tcaaaattag acacaacatt
     1741 gaagatggtg gtgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt
     1801 ccagtcttgt taccagacaa ccattactta tcctatcaat ctgccttatt caaagatcca
     1861 aacgaaaaga gagaccacat ggtcttgtta gaatttttga ctgctgctgg tattaccgag
     1921 ggtatgaatg aattgtacaa aaagaatcaa gcgcatccga ttattgtcgt caaacgacgc
     1981 aaagccaaaa gccacggggc agcacatgga tcgtggaaga ttgcttatgc cgactttatg
     2041 actgcgatga tggccttttt tctggtgatg tggctgatct ccatctccag cccaaaagag
     2101 ctgattcaga ttgcggagta cttccggact ccactggcga ctgcggttac gggcggcgat
     2161 cgcatttcta atagtgaaag cccaattccc ggcggtggtg atgattacac ccaaagccag
     2221 ggggaagtga ataagcagcc gaacatcgaa gagctgaaaa aacgcatgga gcaaagtcga
     2281 ttgcggaaat tgcggggtga tctcgaccag ttgatagagt ccgatccgaa actgcgggcg
     2341 ttacgtcccc atctcaaaat cgatctggtc caggaaggtc tacgtattca gatcatcgat
     2401 agccagaatc gcccgatgtt tagaaccggc agtgccgatg tcgaacccta tatgcgcgac
     2461 attctgcgcg ccattgcgcc tgtactgaac ggtattccca accgtattag cctttcaggt
     2521 cataccgatg atttccccta cgccagcggt gagaaaggat atagcaactg ggagctttct
     2581 gccgatcggg ccaatgcatc ccgccgcgaa ctgatggtcg gagggttgga tagcggcaaa
     2641 gtgttacgtg tcgtcggcat ggcggcaacg atgcgcttaa gcgatcgcgg acctgatgat
     2701 gccgtcaacc gtcgcatcag cctgctggta ctgaacaaac aagccgaaca ggccattttg
     2761 catgaaaacg ccgaaagcca gaatgagcca gtaagcgccc tggaaaaacc tgaggttgca
     2821 ccacaggtca gtgttcccac aatgccatca gccgaaccga ggtgagcagg catgcaagct
     2881 tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag
     2941 aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc
     3001 catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc
     3061 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     3121 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag
     3181 cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcnggacgc ccgccataaa
     3241 ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     3301 aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3361 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3421 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3481 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcag caaactatta actggcgaac
     3541 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3601 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3661 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3721 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3781 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3841 tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3901 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3961 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4021 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     4081 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4141 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     4201 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4261 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat
     4321 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4561 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4621 gcctacatac ctcgctctgc taatcctgtt accagtcagg catttgagaa gcacacggtc
     4681 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     4741 cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc
     4801 gcttattatc acttattcag gcgtagcaac caggcgttta agggcaccaa taactgcctt
     4861 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     4921 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     4981 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     5041 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     5101 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     5161 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     5221 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     5281 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     5341 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     5401 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     5461 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     5521 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     5581 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     5641 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     5701 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
     5761 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     5821 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     5881 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     5941 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     6001 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     6061 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     6121 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     6181 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     6241 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     6301 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     6361 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     6421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     6481 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     6541 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     6601 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     6661 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     6721 agataaaata tttctaggct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     6781 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     6841 cgtccggcgt agaggatctg ctcatgtttg acagcttatc atcgatgcat aatgtgcctg
     6901 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt
     6961 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc
     7021 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag
     7081 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa
     7141 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa
     7201 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct
     7261 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac
     7321 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa
     7381 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt
     7441 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa
     7501 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg
     7561 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat
     7621 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt
     7681 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg
     7741 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag
     7801 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt
     7861 catactcccg ccattcag
//
