LOCUS       sequence-313771-        4842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Chang C, Norris JD, Gron H, Paige LA, Hamilton PT, Kenan DJ, Fowlkes
            D, McDonnell DP
  TITLE     Dissection of the LXXLL nuclear receptor-coactivator interaction 
            motif using combinatorial peptide libraries: discovery of peptide 
            antagonists of estrogen receptors alpha and beta.
  JOURNAL   Mol Cell Biol. 1999 Dec . 19(12):8226-39.
  PUBMED    10567548
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Dec . 19(12):8226-39."
COMMENT     Sequence Label: VP16-ER beta (long)
FEATURES             Location/Qualifiers
     source          1..4842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(29..49)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      357..366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             369..389
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             390..623
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     polyA_signal    2352..2486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2389..2408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2443..2462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2496..2515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2497..2515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2602..2619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2773..3361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2853..2872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3532..4323,4324..4392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4155..4174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4393..4497)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4565..4583
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4621..4643)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4743..4762
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaggtga cactatagaa ctcgatgtgg aatgtgtgtc agttagggtg tggaaagtcc
       61 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaggaaa
      121 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      181 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
      241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
      301 tgagctattc cagaagtagt gaagaggctt ttttggagga gatctaagct agcgccgcca
      361 ccatgggccc taaaaagaag cgtaaagtcg cccccccgac cgatgtcagc ctgggggacg
      421 agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg
      481 atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg
      541 ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc
      601 ttggaattga cgagtacggt ggggaattcc cggggatccg tgatataaaa aactcaccat
      661 ctagccttaa ttctccttcc tcctacaact gcagtcaatc catcttaccc ctggagcacg
      721 gctccatata cataccttcc tcctatgtag acagccacca tgaatatcca gccatgacat
      781 tctatagccc tgctgtgatg aattacagca ttcccagcaa tgtcactaac ttggaaggtg
      841 ggcctggtcg gcagaccaca agcccaaatg tgttgtggcc aacacctggg cacctttctc
      901 ctttagtggt ccatcgccag ttatcacatc tgtatgcgga acctcaaaag agtccctggt
      961 gtgaagcaag atcgctagaa cacaccttac ctgtaaacag agagacactg aaaaggaagg
     1021 ttagtgggaa ccgttgcgcc agccctgtta ctggtccagg ttcaaagagg gatgctcact
     1081 tctgcgctgt ctgcagcgat tacgcatcgg gatatcacta tggagtctgg tcgtgtgaag
     1141 gatgtaaggc cttttttaaa agaagcattc aaggacataa tgattatatt tgtccagcta
     1201 caaatcagtg tacaatcgat aaaaaccggc gcaagagctg ccaggcctgc cgacttcgga
     1261 agtgttacga agtgggaatg gtgaagtgtg gctcccggag agagagatgt gggtaccgcc
     1321 ttgtgcggag acagagaagt gccgacgagc agctgcactg tgccggcaag gccaagagaa
     1381 gtggcggcca cgcgccccga gtgcgggagc tgctgctgga cgccctgagc cccgagcagc
     1441 tagtgctcac cctcctggag gctgagccgc cccatgtgct gatcagccgc cccagtgcgc
     1501 ccttcaccga ggcctccatg atgatgtccc tgaccaagtt ggccgacaag gagttggtac
     1561 acatgatcag ctgggccaag aagattcccg gctttgtgga gctcagcctg ttcgaccaag
     1621 tgcggctctt ggagagctgt tggatggagg tgttaatgat ggggctgatg tggcgctcaa
     1681 ttgaccaccc cggcaagctc atctttgctc cagatcttgt tctggacagg gatgagggga
     1741 aatgcgtaga aggaattctg gaaatctttg acatgctcct ggcaactact tcaaggtttc
     1801 gagagttaaa actccaacac aaagaatatc tctgtgtcaa ggccatgatc ctgctcaatt
     1861 ccagtatgta ccctctggtc acagcgaccc aggatgctga cagcagccgg aagctggctc
     1921 acttgctgaa cgccgtgacc gatgctttgg tttgggtgat tgccaagagc ggcatctcct
     1981 cccagcagca atccatgcgc ctggctaacc tcctgatgct cctgtcccac gtcaggcatg
     2041 cgagtaacaa gggcatggaa catctgctca acatgaagtg caaaaatgtg gtcccagtgt
     2101 atgacctgct gctggagatg ctgaatgccc acgtgcttcg cgggtgcaag tcctccatca
     2161 cggggtccga gtgcagcccg gcagaggaca gtaaaagcaa agagggctcc cagaacccac
     2221 agtctcagtg atctagataa gtaatgatca taatcagcca tatcacatct gtagaggttt
     2281 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2341 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2461 tcaatgtatc ttatcatgtc tggatctgcc ggtctcccta tagtgagtcg tattaatttc
     2521 gataagccag gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2581 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4501 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4561 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4621 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4681 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4741 tgcggcatca gagcagattg tactgagagt gcaccatatg gacatattgt cgttagaacg
     4801 cggctacaat taatacataa ccttatgtat catacacata cg
//
