LOCUS       sequence-313772-        7893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD33 vector encoding MotA, YPet - EAAAK linker - MotB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F
  TITLE     Impact of fluorescent protein fusions on the bacterial flagellar 
            motor.
  JOURNAL   Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w.
  PUBMED    28974721
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-10-3"; volume: "7(1):12583. doi"; pages: " 
            10.1038/s41598-017-11241-w"
COMMENT     Sequence Label: pMH-05
FEATURES             Location/Qualifiers
     source          1..7893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..291
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     214..233
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1232..1945
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     primer_bind     complement(2933..2950)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3103..3189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3281..3308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3327..3418
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3876..4331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3963..3982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4173..4194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4887..5546)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5461..5480
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5547..5649)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6041..6058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6175..6720)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6255..6274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6796..6815
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(6995..7873)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
       61 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
      121 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
      181 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
      241 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
      301 tttgggctag cgaattcgag ctcggtaccc gaaggatgat gtcgtgctta tcttattagg
      361 ttacctggtt gttctcggta cagttttcgg cggttatttg atgaccggtg gaagccttgg
      421 agcactctat caacccgctg aactggtgat tattgccggt gcagggattg ggtcgtttat
      481 cgtcggcaat aatggcaaag cgattaaagg cacgctgaag gcgctgccgt tgctgtttcg
      541 tcgctccaaa tacaccaaag caatgtatat ggatctgctg gctctgcttt atcggttgat
      601 ggcgaaatcg cggcagatgg ggatgttttc gctggaacgt gatattgaaa atccccgtga
      661 gagcgagatc ttcgccagct acccacgcat cctcgcggat agcgtcatgc ttgattttat
      721 cgtcgattat ctgcgcctga ttatcagcgg tcacatgaac accttcgaaa tcgaagctct
      781 gatggatgaa gagattgaga cgcacgaaag cgaggcagaa gtcccggcga acagtctggc
      841 gctggtcggg gactcacttc cggcgtttgg tattgttgcg gctgtaatgg gggtcgttca
      901 cgcgttaggt tcagccgatc gtcctgccgc cgagctgggt gcgcttatcg cacatgcgat
      961 ggtggggact ttcctcggca ttttattggc ttacggattt atttccccat tagcgactgt
     1021 tttacgtcag aaaagcgccg aaaccagcaa aatgatgcag tgcgtcaaag tcactctgct
     1081 ttctaatctg aacggttacg caccgcctat cgccgttgag tttggtcgca aaacgctcta
     1141 ttccagcgaa cgtccgtcgt ttattgaact ggaagagcat gtgcgtgcgg tgaaaaatcc
     1201 gcaacaacag acgacaaccg aggaagcatg aatgtctaaa ggtgaagaat tattcactgg
     1261 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     1321 cggtgaaggt gaaggtgatg ctacgtacgg taaattgacc ttaaaattac tctgtactac
     1381 tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg gtgttcaatg
     1441 ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     1501 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     1561 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     1621 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     1681 ttacatcact gctgacaaac aaaagaatgg tatcaaagct aacttcaaaa ttagacacaa
     1741 cattgaagat ggtggtgttc aattagctga ccattatcaa caaaatactc caattggtga
     1801 tggtccagtc ttgttaccag acaaccatta cttatcctat caatctgcct tattcaaaga
     1861 tccaaacgaa aagagagacc acatggtctt gttagaattt ttgactgctg ctggtattac
     1921 cgagggtatg aatgaattgt acaaagaagc ggcagctaag aagaatcaag cgcatccgat
     1981 tattgtcgtc aaacgacgca aagccaaaag ccacggggca gcacatggat cgtggaagat
     2041 tgcttatgcc gactttatga ctgcgatgat ggcctttttt ctggtgatgt ggctgatctc
     2101 catctccagc ccaaaagagc tgattcagat tgcggagtac ttccggactc cactggcgac
     2161 tgcggttacg ggcggcgatc gcatttctaa tagtgaaagc ccaattcccg gcggtggtga
     2221 tgattacacc caaagccagg gggaagtgaa taagcagccg aacatcgaag agctgaaaaa
     2281 acgcatggag caaagtcgat tgcggaaatt gcggggtgat ctcgaccagt tgatagagtc
     2341 cgatccgaaa ctgcgggcgt tacgtcccca tctcaaaatc gatctggtcc aggaaggtct
     2401 acgtattcag atcatcgata gccagaatcg cccgatgttt agaaccggca gtgccgatgt
     2461 cgaaccctat atgcgcgaca ttctgcgcgc cattgcgcct gtactgaacg gtattcccaa
     2521 ccgtattagc ctttcaggtc ataccgatga tttcccctac gccagcggtg agaaaggata
     2581 tagcaactgg gagctttctg ccgatcgggc caatgcatcc cgccgcgaac tgatggtcgg
     2641 agggttggat agcggcaaag tgttacgtgt cgtcggcatg gcggcaacga tgcgcttaag
     2701 cgatcgcgga cctgatgatg ccgtcaaccg tcgcatcagc ctgctggtac tgaacaaaca
     2761 agccgaacag gccattttgc atgaaaacgc cgaaagccag aatgagccag taagcgccct
     2821 ggaaaaacct gaggttgcac cacaggtcag tgttcccaca atgccatcag ccgaaccgag
     2881 gtgagcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
     2941 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     3001 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     3061 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     3121 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     3181 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     3241 gcannnngcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     3301 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     3361 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3421 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3481 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcagc
     3541 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3601 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3661 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3721 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3781 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3841 gaccaagttt actcatatat actttagatt gatttacgcg ccctgtagcg gcgcattaag
     3901 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3961 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4021 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4081 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4141 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4201 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     4261 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4321 gtttacaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4381 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4441 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4501 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4561 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4621 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtcaggc
     4681 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     4741 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     4801 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcaacc aggcgtttaa
     4861 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcagtactgt
     4921 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     4981 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     5041 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     5101 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     5161 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     5221 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     5281 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa ttccggatga
     5341 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     5401 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     5461 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     5521 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     5581 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     5641 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     5701 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     5761 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     5821 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     5881 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     5941 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     6001 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     6061 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     6121 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     6181 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     6241 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     6301 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     6361 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     6421 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6481 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     6541 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     6601 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     6661 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     6721 gaagatcatc ttattaatca gataaaatat ttctaggctc atgagcccga agtggcgagc
     6781 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     6841 ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga cagcttatca
     6901 tcgatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact
     6961 ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat
     7021 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
     7081 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
     7141 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
     7201 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
     7261 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     7321 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     7381 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     7441 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     7501 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     7561 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     7621 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     7681 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     7741 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     7801 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     7861 gcgcttcagc catacttttc atactcccgc cat
//
