LOCUS       sequence-313773-        7875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD33 vector encoding MotA, Dendra2-MotB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F
  TITLE     Impact of fluorescent protein fusions on the bacterial flagellar 
            motor.
  JOURNAL   Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w.
  PUBMED    28974721
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-10-3"; volume: "7(1):12583. doi"; pages: " 
            10.1038/s41598-017-11241-w"
COMMENT     Sequence Label: pMH-06
FEATURES             Location/Qualifiers
     source          1..7875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..221
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1220..1909
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     primer_bind     complement(2903..2920)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3073..3159
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3251..3278
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3297..3388
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3846..4301
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3933..3952)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4143..4164
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4857..5516)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5431..5450
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5517..5619)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6011..6028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6145..6690)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6225..6244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6766..6785
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(6965..7843)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(7870..7875,1..279)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
       61 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
      121 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
      181 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatccta
      241 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagcg
      301 aattcgagct cggtacccga aggatgatgt cgtgcttatc ttattaggtt acctggttgt
      361 tctcggtaca gttttcggcg gttatttgat gaccggtgga agccttggag cactctatca
      421 acccgctgaa ctggtgatta ttgccggtgc agggattggg tcgtttatcg tcggcaataa
      481 tggcaaagcg attaaaggca cgctgaaggc gctgccgttg ctgtttcgtc gctccaaata
      541 caccaaagca atgtatatgg atctgctggc tctgctttat cggttgatgg cgaaatcgcg
      601 gcagatgggg atgttttcgc tggaacgtga tattgaaaat ccccgtgaga gcgagatctt
      661 cgccagctac ccacgcatcc tcgcggatag cgtcatgctt gattttatcg tcgattatct
      721 gcgcctgatt atcagcggtc acatgaacac cttcgaaatc gaagctctga tggatgaaga
      781 gattgagacg cacgaaagcg aggcagaagt cccggcgaac agtctggcgc tggtcgggga
      841 ctcacttccg gcgtttggta ttgttgcggc tgtaatgggg gtcgttcacg cgttaggttc
      901 agccgatcgt cctgccgccg agctgggtgc gcttatcgca catgcgatgg tggggacttt
      961 cctcggcatt ttattggctt acggatttat ttccccatta gcgactgttt tacgtcagaa
     1021 aagcgccgaa accagcaaaa tgatgcagtg cgtcaaagtc actctgcttt ctaatctgaa
     1081 cggttacgca ccgcctatcg ccgttgagtt tggtcgcaaa acgctctatt ccagcgaacg
     1141 tccgtcgttt attgaactgg aagagcatgt gcgtgcggtg aaaaatccgc aacaacagac
     1201 gacaaccgag gaagcatgaa tgaacacccc gggaattaac ctgatcaagg aggacatgcg
     1261 cgtgaaggtg cacatggagg gcaacgtgaa cggccacgcc ttcgtgatcg agggcgaggg
     1321 caagggcaag ccctacgagg gcacccagac cgccaacctg accgtgaagg agggcgcccc
     1381 cctgcccttc agctacgaca tcctgaccac cgccgtgcac tacggcaacc gggtgttcac
     1441 caagtacccc gaggacatcc ccgactactt caagcagagc ttccccgagg gctacagctg
     1501 ggagcgcacc atgaccttcg aggacaaggg catctgcacc atccgcagcg acatcagcct
     1561 ggagggcgac tgcttcttcc agaacgtgcg cttcaagggc accaacttcc cccccaacgg
     1621 ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc accgagaagc tgcacgtgcg
     1681 cgacggcctg ctggtgggca acatcaacat ggccctgctg ctggagggcg gcggccacta
     1741 cctgtgcgac ttcaagacca cctacaaggc caagaaggtg gtgcagctgc ccgacgccca
     1801 cttcgtggac caccgcatcg agatcctggg caacgacagc gactacaaca aggtgaagct
     1861 gtacgagcac gccgtggccc gctacagccc cctgcccagc caggtgtgga agaatcaagc
     1921 gcatccgatt attgtcgtca aacgacgcaa agccaaaagc cacggggcag cacatggatc
     1981 gtggaagatt gcttatgccg actttatgac tgcgatgatg gccttttttc tggtgatgtg
     2041 gctgatctcc atctccagcc caaaagagct gattcagatt gcggagtact tccggactcc
     2101 actggcgact gcggttacgg gcggcgatcg catttctaat agtgaaagcc caattcccgg
     2161 cggtggtgat gattacaccc aaagccaggg ggaagtgaat aagcagccga acatcgaaga
     2221 gctgaaaaaa cgcatggagc aaagtcgatt gcggaaattg cggggtgatc tcgaccagtt
     2281 gatagagtcc gatccgaaac tgcgggcgtt acgtccccat ctcaaaatcg atctggtcca
     2341 ggaaggtcta cgtattcaga tcatcgatag ccagaatcgc ccgatgttta gaaccggcag
     2401 tgccgatgtc gaaccctata tgcgcgacat tctgcgcgcc attgcgcctg tactgaacgg
     2461 tattcccaac cgtattagcc tttcaggtca taccgatgat ttcccctacg ccagcggtga
     2521 gaaaggatat agcaactggg agctttctgc cgatcgggcc aatgcatccc gccgcgaact
     2581 gatggtcgga gggttggata gcggcaaagt gttacgtgtc gtcggcatgg cggcaacgat
     2641 gcgcttaagc gatcgcggac ctgatgatgc cgtcaaccgt cgcatcagcc tgctggtact
     2701 gaacaaacaa gccgaacagg ccattttgca tgaaaacgcc gaaagccaga atgagccagt
     2761 aagcgccctg gaaaaacctg aggttgcacc acaggtcagt gttcccacaa tgccatcagc
     2821 cgaaccgagg tgaggggatc ctctaggtcg acctgcaggc atgcaagctt ggctgttttg
     2881 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     2941 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     3001 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     3061 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3121 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     3181 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     3241 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     3301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3361 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3421 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3481 aaaagatgct gaagatcagt tgggtgcagc aaactattaa ctggcgaact acttactcta
     3541 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3601 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3661 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3721 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3781 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3841 gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3901 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3961 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4021 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4081 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4141 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
     4201 tttataagtg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4261 atttaacgcg aattttaaca aaatattaac gtttacaatt taaaaggatc taggtgaaga
     4321 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4381 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4441 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4501 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4561 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4621 tcgctctgct aatcctgtta ccagtcaggc atttgagaag cacacggtca cactgcttcc
     4681 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc
     4741 tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
     4801 cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac
     4861 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     4921 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     4981 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     5041 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     5101 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     5161 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     5221 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     5281 tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     5341 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     5401 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     5461 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     5521 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     5581 tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag
     5641 ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga
     5701 tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg
     5761 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
     5821 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
     5881 ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag tgaagtgctt
     5941 catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat
     6001 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     6061 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     6121 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     6181 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6241 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     6301 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     6361 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     6421 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     6481 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     6541 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     6601 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     6661 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
     6721 ttctaggctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
     6781 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     6841 gaggatctgc tcatgtttga cagcttatca tcgatgcata atgtgcctgt caaatggacg
     6901 aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta
     6961 ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact
     7021 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa
     7081 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg
     7141 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga
     7201 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa
     7261 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca
     7321 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca
     7381 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc
     7441 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg
     7501 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc
     7561 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg
     7621 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc
     7681 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa
     7741 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa
     7801 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc
     7861 cattcagaga agaaa
//
