LOCUS       sequence-313777-        7896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD33 vector encoding MotA, Dendra2 - GSGSGS linker - MotB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F
  TITLE     Impact of fluorescent protein fusions on the bacterial flagellar 
            motor.
  JOURNAL   Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w.
  PUBMED    28974721
REFERENCE   2  (bases 1 to 7896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-10-3"; volume: "7(1):12583. doi"; pages: " 
            10.1038/s41598-017-11241-w"
COMMENT     Sequence Label: pMH-09
FEATURES             Location/Qualifiers
     source          1..7896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..310
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     233..252
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1251..1940
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     primer_bind     complement(2955..2972)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3125..3211
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3303..3330
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3349..3440
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3898..4353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3985..4004)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4195..4216
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4909..5568)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5483..5502
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5569..5671)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6063..6080)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6197..6742)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6277..6296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6818..6837
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(7017..7895)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
       61 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa
      121 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
      181 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
      241 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
      301 gtttctccat acccgttttt ttgggctagc gaattcgagc tcggtacccg aaggatgatg
      361 tcgtgcttat cttattaggt tacctggttg ttctcggtac agttttcggc ggttatttga
      421 tgaccggtgg aagccttgga gcactctatc aacccgctga actggtgatt attgccggtg
      481 cagggattgg gtcgtttatc gtcggcaata atggcaaagc gattaaaggc acgctgaagg
      541 cgctgccgtt gctgtttcgt cgctccaaat acaccaaagc aatgtatatg gatctgctgg
      601 ctctgcttta tcggttgatg gcgaaatcgc ggcagatggg gatgttttcg ctggaacgtg
      661 atattgaaaa tccccgtgag agcgagatct tcgccagcta cccacgcatc ctcgcggata
      721 gcgtcatgct tgattttatc gtcgattatc tgcgcctgat tatcagcggt cacatgaaca
      781 ccttcgaaat cgaagctctg atggatgaag agattgagac gcacgaaagc gaggcagaag
      841 tcccggcgaa cagtctggcg ctggtcgggg actcacttcc ggcgtttggt attgttgcgg
      901 ctgtaatggg ggtcgttcac gcgttaggtt cagccgatcg tcctgccgcc gagctgggtg
      961 cgcttatcgc acatgcgatg gtggggactt tcctcggcat tttattggct tacggattta
     1021 tttccccatt agcgactgtt ttacgtcaga aaagcgccga aaccagcaaa atgatgcagt
     1081 gcgtcaaagt cactctgctt tctaatctga acggttacgc accgcctatc gccgttgagt
     1141 ttggtcgcaa aacgctctat tccagcgaac gtccgtcgtt tattgaactg gaagagcatg
     1201 tgcgtgcggt gaaaaatccg caacaacaga cgacaaccga ggaagcatga atgaacaccc
     1261 cgggaattaa cctgatcaag gaggacatgc gcgtgaaggt gcacatggag ggcaacgtga
     1321 acggccacgc cttcgtgatc gagggcgagg gcaagggcaa gccctacgag ggcacccaga
     1381 ccgccaacct gaccgtgaag gagggcgccc ccctgccctt cagctacgac atcctgacca
     1441 ccgccgtgca ctacggcaac cgggtgttca ccaagtaccc cgaggacatc cccgactact
     1501 tcaagcagag cttccccgag ggctacagct gggagcgcac catgaccttc gaggacaagg
     1561 gcatctgcac catccgcagc gacatcagcc tggagggcga ctgcttcttc cagaacgtgc
     1621 gcttcaaggg caccaacttc ccccccaacg gccccgtgat gcagaagaag accctgaagt
     1681 gggagcccag caccgagaag ctgcacgtgc gcgacggcct gctggtgggc aacatcaaca
     1741 tggccctgct gctggagggc ggcggccact acctgtgcga cttcaagacc acctacaagg
     1801 ccaagaaggt ggtgcagctg cccgacgccc acttcgtgga ccaccgcatc gagatcctgg
     1861 gcaacgacag cgactacaac aaggtgaagc tgtacgagca cgccgtggcc cgctacagcc
     1921 ccctgcccag ccaggtgtgg ggtagcggta gcggtagcat gaagaatcaa gcgcatccga
     1981 ttattgtcgt caaacgacgc aaagccaaaa gccacggggc agcacatgga tcgtggaaga
     2041 ttgcttatgc cgactttatg actgcgatga tggccttttt tctggtgatg tggctgatct
     2101 ccatctccag cccaaaagag ctgattcaga ttgcggagta cttccggact ccactggcga
     2161 ctgcggttac gggcggcgat cgcatttcta atagtgaaag cccaattccc ggcggtggtg
     2221 atgattacac ccaaagccag ggggaagtga ataagcagcc gaacatcgaa gagctgaaaa
     2281 aacgcatgga gcaaagtcga ttgcggaaat tgcggggtga tctcgaccag ttgatagagt
     2341 ccgatccgaa actgcgggcg ttacgtcccc atctcaaaat cgatctggtc caggaaggtc
     2401 tacgtattca gatcatcgat agccagaatc gcccgatgtt tagaaccggc agtgccgatg
     2461 tcgaacccta tatgcgcgac attctgcgcg ccattgcgcc tgtactgaac ggtattccca
     2521 accgtattag cctttcaggt cataccgatg atttccccta cgccagcggt gagaaaggat
     2581 atagcaactg ggagctttct gccgatcggg ccaatgcatc ccgccgcgaa ctgatggtcg
     2641 gagggttgga tagcggcaaa gtgttacgtg tcgtcggcat ggcggcaacg atgcgcttaa
     2701 gcgatcgcgg acctgatgat gccgtcaacc gtcgcatcag cctgctggta ctgaacaaac
     2761 aagccgaaca ggccattttg catgaaaacg ccgaaagcca gaatgagcca gtaagcgccc
     2821 tggaaaaacc tgaggttgca ccacaggtca gtgttcccac aatgccatca gccgaaccga
     2881 ggtgagggga tcctctaggt cgacctgcag gcatgcaagc ttggctgttt tggcggatga
     2941 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag
     3001 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg
     3061 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
     3121 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     3181 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     3241 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     3301 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     3361 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3421 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3481 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3541 ctgaagatca gttgggtgca gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3601 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3661 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3721 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3781 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3841 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttacg
     3901 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3961 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4021 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4081 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     4141 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4201 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     4261 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4321 cgaattttaa caaaatatta acgtttacaa tttaaaagga tctaggtgaa gatccttttt
     4381 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4441 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4501 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4561 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4621 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4681 ctaatcctgt taccagtcag gcatttgaga agcacacggt cacactgctt ccggtagtca
     4741 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
     4801 cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat cacttattca
     4861 ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc
     4921 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     4981 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     5041 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     5101 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     5161 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     5221 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     5281 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     5341 tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     5401 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     5461 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     5521 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     5581 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     5641 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca
     5701 gggcttcccg gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt
     5761 cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct gccaacttac tgatttagtg
     5821 tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt
     5881 cagctactga cggggtggta cgtaacggca aaagcaccgc cggacatcag cgctagcgga
     5941 gtgtatactg gcttactatg ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc
     6001 aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat atattccgct
     6061 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga aatggcttac
     6121 gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga agtgagaggg
     6181 ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac
     6241 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6301 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     6361 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     6421 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     6481 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     6541 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     6601 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     6661 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     6721 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctaggc
     6781 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     6841 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct
     6901 gctcatgttt gacagcttat catcgatgca taatgtgcct gtcaaatgga cgaagcaggg
     6961 attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat
     7021 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
     7081 ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca
     7141 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
     7201 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
     7261 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
     7321 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
     7381 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
     7441 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
     7501 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
     7561 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
     7621 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
     7681 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
     7741 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
     7801 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
     7861 cccggcagca ggggatcatt ttgcgcttca gccata
//
