LOCUS       sequence-313778-        7947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBAD33 vector encoding MotA, YPet - 3 x EAAAK linker - MotB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Heo M, Nord AL, Chamousset D, van Rijn E, Beaumont HJE, Pedaci F
  TITLE     Impact of fluorescent protein fusions on the bacterial flagellar 
            motor.
  JOURNAL   Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.1038/s41598-017-11241-w.
  PUBMED    28974721
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-10-3"; volume: "7(1):12583. doi"; pages: " 
            10.1038/s41598-017-11241-w"
COMMENT     Sequence Label: pMH-25
FEATURES             Location/Qualifiers
     source          1..7947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..293
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     216..235
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1234..1947
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
     primer_bind     complement(2989..3006)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3159..3245
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3337..3364
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3383..3474
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3932..4387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4019..4038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4229..4250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4943..5602)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5517..5536
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(5603..5705)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6097..6114)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6231..6776)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6311..6330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6852..6871
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(7051..7929)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
       61 ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
      121 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
      181 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
      241 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
      301 tttttgggct agcgaattcg agctcggtac ccgaaggatg atgtcgtgct tatcttatta
      361 ggttacctgg ttgttctcgg tacagttttc ggcggttatt tgatgaccgg tggaagcctt
      421 ggagcactct atcaacccgc tgaactggtg attattgccg gtgcagggat tgggtcgttt
      481 atcgtcggca ataatggcaa agcgattaaa ggcacgctga aggcgctgcc gttgctgttt
      541 cgtcgctcca aatacaccaa agcaatgtat atggatctgc tggctctgct ttatcggttg
      601 atggcgaaat cgcggcagat ggggatgttt tcgctggaac gtgatattga aaatccccgt
      661 gagagcgaga tcttcgccag ctacccacgc atcctcgcgg atagcgtcat gcttgatttt
      721 atcgtcgatt atctgcgcct gattatcagc ggtcacatga acaccttcga aatcgaagct
      781 ctgatggatg aagagattga gacgcacgaa agcgaggcag aagtcccggc gaacagtctg
      841 gcgctggtcg gggactcact tccggcgttt ggtattgttg cggctgtaat gggggtcgtt
      901 cacgcgttag gttcagccga tcgtcctgcc gccgagctgg gtgcgcttat cgcacatgcg
      961 atggtgggga ctttcctcgg cattttattg gcttacggat ttatttcccc attagcgact
     1021 gttttacgtc agaaaagcgc cgaaaccagc aaaatgatgc agtgcgtcaa agtcactctg
     1081 ctttctaatc tgaacggtta cgcaccgcct atcgccgttg agtttggtcg caaaacgctc
     1141 tattccagcg aacgtccgtc gtttattgaa ctggaagagc atgtgcgtgc ggtgaaaaat
     1201 ccgcaacaac agacgacaac cgaggaagca tgaatgtcta aaggtgaaga attattcact
     1261 ggtgttgtcc caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc
     1321 tccggtgaag gtgaaggtga tgctacgtac ggtaaattga ccttaaaatt actctgtact
     1381 actggtaaat tgccagttcc atggccaacc ttagtcacta ctttaggtta tggtgttcaa
     1441 tgttttgcta gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca
     1501 gaaggttatg ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga
     1561 gctgaagtca agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat
     1621 tttaaagaag atggtaacat tttaggtcac aaattggaat acaactataa ctctcacaat
     1681 gtttacatca ctgctgacaa acaaaagaat ggtatcaaag ctaacttcaa aattagacac
     1741 aacattgaag atggtggtgt tcaattagct gaccattatc aacaaaatac tccaattggt
     1801 gatggtccag tcttgttacc agacaaccat tacttatcct atcaatctgc cttattcaaa
     1861 gatccaaacg aaaagagaga ccacatggtc ttgttagaat ttttgactgc tgctggtatt
     1921 accgagggta tgaatgaatt gtacaaagaa gcggcagcta aggaagcggc agctaaggaa
     1981 gcggcagcta agatgaagaa tcaagcgcat ccgattattg tcgtcaaacg acgcaaagcc
     2041 aaaagccacg gggcagcaca tggatcgtgg aagattgctt atgccgactt tatgactgcg
     2101 atgatggcct tttttctggt gatgtggctg atctccatct ccagcccaaa agagctgatt
     2161 cagattgcgg agtacttccg gactccactg gcgactgcgg ttacgggcgg cgatcgcatt
     2221 tctaatagtg aaagcccaat tcccggcggt ggtgatgatt acacccaaag ccagggggaa
     2281 gtgaataagc agccgaacat cgaagagctg aaaaaacgca tggagcaaag tcgattgcgg
     2341 aaattgcggg gtgatctcga ccagttgata gagtccgatc cgaaactgcg ggcgttacgt
     2401 ccccatctca aaatcgatct ggtccaggaa ggtctacgta ttcagatcat cgatagccag
     2461 aatcgcccga tgtttagaac cggcagtgcc gatgtcgaac cctatatgcg cgacattctg
     2521 cgcgccattg cgcctgtact gaacggtatt cccaaccgta ttagcctttc aggtcatacc
     2581 gatgatttcc cctacgccag cggtgagaaa ggatatagca actgggagct ttctgccgat
     2641 cgggccaatg catcccgccg cgaactgatg gtcggagggt tggatagcgg caaagtgtta
     2701 cgtgtcgtcg gcatggcggc aacgatgcgc ttaagcgatc gcggacctga tgatgccgtc
     2761 aaccgtcgca tcagcctgct ggtactgaac aaacaagccg aacaggccat tttgcatgaa
     2821 aacgccgaaa gccagaatga gccagtaagc gccctggaaa aacctgaggt tgcaccacag
     2881 gtcagtgttc ccacaatgcc atcagccgaa ccgaggtgag gggatcctct aggtcgacct
     2941 gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
     3001 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     3061 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3121 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     3181 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     3241 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     3301 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     3361 tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg
     3421 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3481 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3541 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcagcaaac
     3601 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3661 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3721 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3781 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3841 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3901 aagtttactc atatatactt tagattgatt tacgcgccct gtagcggcgc attaagcgcg
     3961 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4021 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4081 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4141 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4201 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4261 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4321 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4381 acaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4621 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tcaggcattt
     4741 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     4801 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     4861 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc
     4921 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta
     4981 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     5041 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     5101 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     5161 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     5221 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     5281 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     5341 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     5401 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     5461 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     5521 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     5581 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     5641 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     5701 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     5761 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     5821 cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
     5881 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
     5941 ggcaaaagca ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca
     6001 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     6061 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     6121 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     6181 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     6241 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     6301 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     6361 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     6421 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     6481 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     6541 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     6601 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     6661 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     6721 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     6781 atcatcttat taatcagata aaatatttct aggctcatga gcccgaagtg gcgagcccga
     6841 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     6901 atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc ttatcatcga
     6961 tgcataatgt gcctgtcaaa tggacgaagc agggattctg caaaccctat gctactccgt
     7021 caagccgtca attgtctgat tcgttaccaa ttatgacaac ttgacggcta catcattcac
     7081 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
     7141 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
     7201 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
     7261 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
     7321 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
     7381 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
     7441 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
     7501 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
     7561 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
     7621 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
     7681 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
     7741 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
     7801 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
     7861 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
     7921 ttcagccata cttttcatac tcccgcc
//
