LOCUS       sequence-31379-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CBX6
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             294..311
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(364..382)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      378..425
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      462..917
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(549..568)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     759..780
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt gagcgggtct tcgcggccga atccatcatc
      121 aaacggcgga tccgaaaggg acgcatcgag tacctggtga aatggaaggg gtgggcgatc
      181 aagtacagca cttgggagcc cgaggagaac atcctggact cgcggctcat tgcagccttc
      241 gaacaaaagg agagggagcg tgagtgatga cgaagcttgc ggccgcactc gagcaccacc
      301 accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      361 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      421 ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc
      481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      661 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      781 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      901 attaacgctt acaatttaag tg
//
