LOCUS       sequence-313794-        6221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Macias-Silva M, Hoodless PA, Tang SJ, Buchwald M, Wrana JL
  TITLE     Specific activation of Smad1 signaling pathways by the BMP7 type I 
            receptor, ALK2.
  JOURNAL   J Biol Chem. 1998 Oct 2. 273(40):25628-36.
  PUBMED    9748228
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Oct 2. 273(40):25628-36."
COMMENT     Sequence Label: pCMV5-ActRIIB
FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2245..2867
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2444..2463)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2891..2911)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2896..3225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3076..3211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3263..3282)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3264..3282)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3296..3312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3296..3312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3309..3331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3320..3336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3344..3350,3351..3368,3369..3374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3389..3410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3527..3544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3698..4286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3778..3797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4457..5248,5249..5317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5080..5099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5318..5422)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5449..5904
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5536..5555)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5746..5767
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6030..6052
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6044..6061
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6045..6061
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcgcg gaacatgacg gcgccctggg cggccctcgc ccttctctgg ggatcgctgt
      661 gcgccggttc cgggcgaggg gaggctgaga ctcgggagtg catctactac aacgccaact
      721 gggagctgga gcgcaccaac cagagcggcc tggagcgctg cgagggggaa caggacaagc
      781 ggctgcactg ctacgcctcg tggcgcaaca gctcgggcac catcgagctg gtgaagaagg
      841 gctgctggct agatgacttc aattgctacg acaggcagga gtgtgtggcc accgaggaga
      901 acccccaggt gtacttctgc tgctgcgaag gcaacttctg caacgagcgc ttcacccact
      961 tgccggagcc tgggggccca gaagtcacgt acgagccacc cccgacagcc cccaccctgc
     1021 tcacggtgct ggcctactcg ctgctgccca ttggaggcct ctctctcatc gtcctgctgg
     1081 ccttctggat gtatcgtcat cggaaacctc cctacggcca tgtggacatc catgaggacc
     1141 cggggcctcc gcccccatcc cctctggtgg gcctgaagcc actacagctg ctggagatca
     1201 aggctcgggg ccgctttggc tgcgtttgga aggctcagct catgaacgac tttgtggctg
     1261 tgaagatctt cccacttcag gacaagcagt cgtggcagag tgaacgggaa atcttcagca
     1321 cacccggcat gaagcacgaa aacttgttgc agttcattgc tgccgagaaa cgaggctcca
     1381 acctggaggt ggagctgtgg ctcatcacag ccttccacga caagggctcc ctcacggatt
     1441 acctcaaggg gaacatcatc acgtggaacg aactgtgcca cgtggcggag acgatgtcac
     1501 gaggcctctc atacctgcat gaggatgtgc cgtggtgtcg tggtgagggc cacaagcctt
     1561 ctattgccca cagggacttc aaaagcaaga atgtactgct gaagagcgac ctcaccgcgg
     1621 tgctggctga cttcggcctg gctgttcggt ttgagccagg gaagcctcct ggggataccc
     1681 atggacaggt tggcaccaga cggtacatgg cccctgaggt gctggaagga gccatcaact
     1741 tccagagaga cgccttcctg cgtatcgaca tgtacgccat gggcctggtg ctgtgggagc
     1801 tcgtctctcg gtgcaaggct gcagacgggc ctgtcgatga gtacatgctg cccttcgagg
     1861 aggagattgg ccagcaccct tcgctggagg agcttcagga ggtggttgtc cacaagaaga
     1921 tgaggcccac gattaaggat cactggctga aacacccggg cctggcccag ctctgcgtga
     1981 ccatcgagga gtgctgggac catgatgcag aggctcgcct ttctgcaggc tgtgtagaag
     2041 agcgggtatc cctgatcagg aggtcggtca acggcactac ctcggactgt ctcgtctctc
     2101 tggtgacctc cgtcaccaat gtggacctgc tccctaaaga gtccagcatc taagcccggg
     2161 acacgtagcg tctctccaga ctcagtggat ctgaagaaaa aaaaaagctt tagagcacac
     2221 tggcggccgt tactagtgga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     2281 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     2341 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     2401 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     2461 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     2521 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     2581 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     2641 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     2701 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     2761 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     2821 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     2881 gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2941 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3061 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3181 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cctcgaggaa
     3241 ctgaaaaacc agaaagttaa ttccctatag tgagtcgtat taaattcgta atcatgtcat
     3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
     5461 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5521 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5581 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5641 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5701 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5761 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5821 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     5881 acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg
     5941 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     6001 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     6061 tgccaagctg atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta
     6121 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
     6181 gtacatttat attggctcat gtccaatatg accgccatgt t
//
