LOCUS       sequence-313796-       10344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Luo JH, Fu ZY, Losi G, Kim BG, Prybylowski K, Vissel B, Vicini S
  TITLE     Functional expression of distinct NMDA channel subunits tagged with 
            green fluorescent protein in hippocampal neurons in culture.
  JOURNAL   Neuropharmacology. 2002 Mar . 42(3):306-18.
  PUBMED    11897109
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2002 Mar . 42(3):306-18."
COMMENT     Sequence Label: pEYFP-NR1a
FEATURES             Location/Qualifiers
     source          1..10344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1037..1039,1040..1042,1043..1753)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1082..1103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1343..1362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1690..1711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5701..5718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5707..5931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5977..6405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6064..6083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6274..6295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6414..6434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6419..6748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6599..6734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6661..6680
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6815..7609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6869..6888)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7479..7498
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7783..7904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7820..7839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7874..7893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7953..7969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7953..7969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7966..7988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7977..7993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8001..8007,8008..8025,8026..8031))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8046..8067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8184..8201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8355..8943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8435..8454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9114..9905,9906..9974))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9737..9756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9975..10079)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10154..10173)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgatatcga attccgttgc tgtcgaatat tggtttgtta
      721 aggcagtttc tgtagaggtt tctaagagac cagtcgcgca gtccgcgctg ctgtcctttc
      781 cgccttttcc gcgcgggtgt tcgagcagcg ccaaacacgc ttcagcacct cggacagcat
      841 ccgccgcgct cgcccggggc tcctagagaa cccgggggcg cttggccgcg cgcgggcggc
      901 ccgcgggtcg tacatcgcga ggtcgtcgca ctcgcgcaac ccagagccag gcccgctgtg
      961 cccggagctc atgagcacca tgcacctgct gacattcgcc ctgctttttt cctgctcctt
     1021 cgcccgcgcg gatcccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1081 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1141 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1201 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta
     1261 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1321 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1381 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1441 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     1501 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1561 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1621 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     1681 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1741 cgagctgtac aaggatcccg accccaagat cgtcaacatc ggcgcggtgc tgagcacgcg
     1801 taagcatgaa cagatgttcc gcgaggcagt aaaccaggcc aataagcgac acggctcttg
     1861 gaagatacag ctcaacgcca cttctgtcac ccacaagccc aacgccatac agatggccct
     1921 gtcagtgtgt gaggacctca tctctagcca ggtctacgct atcctagtta gccacccgcc
     1981 tactcccaac gaccacttca ctcccacccc tgtctcctac acagctggct tctacagaat
     2041 ccctgtcctg ggactgacta cccgaatgtc catctactct gacaagagta tccacctgag
     2101 tttccttcgc acggtgccgc cctactccca ccagtccagc gtctggtttg agatgatgcg
     2161 agtctacaac tggaaccaca tcatcctgct ggtcagcgac gaccacgagg gacgggcagc
     2221 gcagaagcgc ttggagacgt tgctggagga acgggagtcc aaggcagaga aggtgctgca
     2281 gtttgaccca ggaaccaaga atgtgacggc tctgctgatg gaggcccggg aactggaggc
     2341 ccgggtcatc atcctttctg caagcgagga cgacgctgcc acagtgtacc gcgcagccgc
     2401 aatgctgaac atgacgggct ctgggtacgt gtggctggtc ggggaacgcg agatctctgg
     2461 gaacgccctg cgctacgctc ctgatggcat catcggactt cagctcatca atggcaagaa
     2521 tgagtcagcc cacatcagtg acgccgtggg cgtggtggca caggcagttc acgaactcct
     2581 agagaaggag aatatcactg acccaccgcg gggttgcgtg ggcaacacca acatctggaa
     2641 gacaggacca ttgttcaaga gggtgctgat gtcttctaag tatgcggacg gagtgactgg
     2701 ccgtgtggaa ttcaatgagg atggggaccg gaagtttgcc aactatagta tcatgaacct
     2761 gcagaaccgc aagctggtgc aagtgggcat ctacaatggt acccatgtca tcccaaatga
     2821 caggaagatc atctggccag gaggagagac agagaaacct cgaggatacc agatgtccac
     2881 cagactaaag atagtgacaa tccaccaaga gcccttcgtg tacgtcaagc ccacaatgag
     2941 tgatgggaca tgcaaagagg agttcacagt caatggtgac ccagtgaaga aggtgatctg
     3001 tacggggcct aatgacacgt ccccaggcag cccacgccac acagtgcccc agtgctgcta
     3061 tggcttctgc atagacctgc tcatcaagct ggcgcggacc atgaatttta cctatgaggt
     3121 gcacctggtg gcagatggca agtttggcac acaggagcgg gtaaacaaca gcaacaaaaa
     3181 ggagtggaac ggaatgatgg gcgagctact cagtggccaa gcggacatga ttgtggcacc
     3241 actgaccatc aacaatgagc gtgcgcagta catagagttc tccaagccct tcaagtacca
     3301 gggcctgacc attttggtca agaaggagat tcccaggagc acactggact catttatgca
     3361 gccttttcag agcacactgt ggttgctagt aggactgtca gttcatgtgg tggctgtgat
     3421 gctgtacctg ctggaccgct tcagtccctt tggccgattc aaggtgaaca gtgaggagga
     3481 ggaggaagat gcactgaccc tgtcctctgc catgtggttt tcctggggcg tcctgctcaa
     3541 ctccggcatt ggggaaggtg ccccccggag tttctctgca cgtatcctag gcatggtgtg
     3601 ggctggtttc gccatgatca tagtggcttc ctacactgcc aacttggcag ctttcctggt
     3661 gctggatcgg cctgaggagc gcatcacggg catcaatgac cccaggctca gaaacccctc
     3721 agacaagttc atctacgcaa ctgtaaagca gagctccgtg gacatctact tccggaggca
     3781 ggtggagttg agtaccatgt accggcacat ggaaaaacac aattacgaga gcgcagctga
     3841 ggccatccag gctgtgcggg acaacaagct gcacgccttt atctgggact cggccgtgct
     3901 ggagtttgag gcttcacaga agtgcgatct ggtgaccacg ggtgagctgt tcttccgctc
     3961 aggctttggc atcggcatgc gcaaggacag cccctggaag cagaacgttt ccctgtccat
     4021 actcaagtcc catgagaatg gcttcatgga agatctggat aagacatggg ttcggtatca
     4081 ggaatgcgac tcccgcagca atgctcctgc aaccctcact tttgagaaca tggcaggggt
     4141 cttcatgctg gtggctggag gcatcgtagc tgggattttc ctcattttca ttgagatcgc
     4201 ctacaagcga cacaaggatg cccgtaggaa gcagatgcag ctggcttttg cagccgtgaa
     4261 cgtgtggagg aagaacctgc aggatagaaa gagtggtaga gcagagcccg accctaaaaa
     4321 gaaagccaca tttagggcta tcacctccac cctggcctcc agcttcaaga gacgtaggtc
     4381 ctccaaagac acgagcaccg ggggtggacg cggcgctttg caaaaccaaa aagacacagt
     4441 gctgccgcga cgcgctattg agagggagga gggccagctg cagctgtgtt cccgtcatag
     4501 ggagagctga gacgccccgc ccgccctcct ctgcccctcc cccgcagaca gacgcacggg
     4561 acagcggcct ggcccacgca gagccccgga gcacgacggg gtcgggggag gagcactccc
     4621 agcctccccc aggccgtgcc cgcctgccca ccggtcggcc ggctggccgg tccaccctgt
     4681 cccggccccg cgcgtgcccc cgacgtcgga gctaacgggc cgccttgtct gtgtatttct
     4741 attttacagc agtaccatcc cactgatatc acgggcccgc tcaacctctc agatccctcg
     4801 gtcagcaccg tggtgtgagg ccccccggag gcgcccacct gcccagttag cccggccaag
     4861 gacactgatg agtcctgctg ctcgggaagg cctgagggaa gcccacccgc cccagagact
     4921 gcccaccctg ggcctcccgt ccgcctgctc tgctgcctgg cgggcagccc ctgcaggacc
     4981 aaggtgcgga ccagagcggc tgaggatggg ccagagctga gccggctggg cagggccaca
     5041 gggcgctccg gcagaggcag ggccctgagg tctctgagca gtggggtgag gggcctaagt
     5101 ggccccggtc ggaggagtct ggagcagaaa tggcagcccc atccttcctc cagccactac
     5161 cccaagctac agtgggggcc tatggcccca gcttgctagg tcacccccga cccttcctcc
     5221 agcgcctgct ctctgcaact tgatttccac ctctctcctg ctgcaccacc ctcccacgac
     5281 atttccccac cccattcact gggttgtctc tgacctttcc cagggctagc cttcactgcc
     5341 ctagtggcag tgcttcaggg gtgctttctg gctcccagac atctagggct ccagactcca
     5401 agagggctga gccttctctt ctgtccgcag ccacaatagg cttcctcaga cgctggctcg
     5461 tgatgagtcc cgcaccttgg gcaccaggga gcgccatctg cctcccagtc cggtgtcact
     5521 caccccacta ccttgtacat gaccagctct cccagtgtcc cagtgtctgc cccagggaca
     5581 ccgggcgcgc acagccaccc ctaatcccgg tattcagtgg tgatgcctaa aggaatgtca
     5641 gaaaaaaaaa aaaaaagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
     5701 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5761 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5821 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5881 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5941 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6001 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6061 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6421 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6481 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6541 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6601 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6661 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6721 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6781 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6841 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6901 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6961 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     7021 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     7081 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     7141 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     7201 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     7261 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     7321 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     7381 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7441 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7501 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7561 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     7621 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     7681 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7741 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     7801 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7861 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     7921 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7981 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8041 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     8101 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     8161 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8221 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8281 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8341 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8401 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8461 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8521 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8581 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8641 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8701 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8761 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8821 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8881 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8941 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9001 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9061 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9121 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9181 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9241 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9301 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9361 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9421 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9481 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9541 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9601 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9661 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9721 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9781 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9841 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9901 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9961 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10021 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10081 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    10141 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    10201 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    10261 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
    10321 atgtacgggc cagatatacg cgtt
//
