LOCUS       sequence-31380-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HDAC6 (3GV4)
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             441..458
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(511..529)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      525..572
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(696..715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     906..927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt ccactgccct ggtgtcccca tttggtggca
      121 gtatgcccca tacctgcagc aggcctagac gtgacccaac cttgtgggga ctgtggaaca
      181 atccaagaga attgggtgtg tctctcttgc tatcaggtct actgtggtcg ttacatcaat
      241 ggccacatgc tccaacacca tggaaattct ggacacccgc tggtcctcag ctacatcgac
      301 ctgtcagcct ggtgttacta ctgtcaggcc tatgtccacc accaggctct cctagatgtg
      361 aagaacatcg cccaccagaa caagtttggg gaggatatgc cccacccaca ctgatgacga
      421 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      481 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      541 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      601 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      841 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      901 taatagtgga ctcttgttcc aaactggaac aacactcaac ct
//
