LOCUS       sequence-31381-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RNF168
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             469..486
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(539..557)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      553..600
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(724..743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt atggctctac ccaaagacgc
      121 catcccctcg ctgtccgagt gccagtgcgg gatctgcatg gaaatcctcg tggagcccgt
      181 caccctcccg tgtaaccaca cgctgtgtaa accgtgcttc cagtcgaccg tcgaaaaggc
      241 gagtttatgc tgtcccttct gtcgccgccg ggtatcgtcg tggactcggt accatacccg
      301 aagaaattct ctcgtcaacg tggaactgtg gacgataatt caaaaacact atcccaggga
      361 gtgcaagctt agagcgtctg gccaagaatc agaggaagtg gctgatgact atcagccagt
      421 tcgtctgctc agtaaacctt gatgacgaag cttgcggccg cactcgagca ccaccaccac
      481 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      541 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      601 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      661 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      721 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      781 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      841 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      901 gcccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcaa t
//
