LOCUS       sequence-313817-        6017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RPU standard, pNBU1 backbone with BsaI sites, AmpR, ErmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Taketani M, Zhang J, Zhang S, Triassi AJ, Huang YJ, Griffith LG, 
            Voigt CA
  TITLE     Genetic circuit design automation for the gut resident species 
            Bacteroides thetaiotaomicron.
  JOURNAL   Nat Biotechnol. 2020 Aug;38(8):962-969. doi: 
            10.1038/s41587-020-0468-5. Epub 2020 Mar 30.
  PUBMED    32231334
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0468-5"; journalName: "Nat Biotechnol"; date: 
            "2020-08"; volume: "38"; issue: "8"; pages: "962-969"
COMMENT     Sequence Label: pMT406
FEATURES             Location/Qualifiers
     source          1..6017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1484..1552,1553..2344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1702..1721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            2475..2584
                     /note="incP origin of transfer"
     rep_origin      2755..3143
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     misc_RNA        3525..3574
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 gtatttgata gttcaatact tagctttttc ttttgcttga attttcccca cattttcccc
       61 acacgtgcaa aaaatatagc agtaagtcat tatttctttt ggttgaacgt agagagtagc
      121 gatattaaaa gaatccgatg agaaaagact aatatttatc tatccattca gtttgatttc
      181 tcaggacttt acatcgtcct gaaagtattt gttggtaccg aggacgcgta aacatttaca
      241 gttgcatgtg gcctattgtt tttagccgtt aaatatttta taactattaa atagcgatac
      301 aaattgttcg aaactaatat tgtttatatc atatattctc gcatgtttta aagctttatt
      361 aaattgattt tttgtaaaca gtttttcgta ctctttgtta acccatttca ttacaaaagt
      421 ttcatatttt tttctctctt taaatgccat ttttgctggc tttcttttta atacaattaa
      481 tgtgctatcc actttaggtt ttggatggaa ataataccta ggaatttttg ctaatataga
      541 aatatctacc tctgccatta acagcaatgc tagtgatctg tttgtatcta ataacatttt
      601 agcaaaacca tattccacta ttaaataact tattgtggct gaactttcaa aaacaatttt
      661 tcgaattata tttgtgctta tgttgtaagg tatgctgcca aatattttat atggattgtg
      721 gctaggaaat gtaaatttca gtatatcatc atttactatt tgatagttag gataatttaa
      781 gagcttatta cgagttacct cacataattt agaatcaatt tctatcgccg ttacaaaatt
      841 acatctcttt accaatccag cagtaaaatg acctttccct gcacctattt caaagatgtt
      901 atctttttca tctaaactta tgcaattcat tattttttct atgtgatatt ttgaagtaat
      961 aaaattttga ctatctttta tatttacttt gttcattata acctctcctt aatttattgc
     1021 atctcttttc gaatatttat gttttttgag aaaagaacgt actcatggtt catcccgata
     1081 tgcgtatcgg tctgtatatc agcaactttc tatgtgtttc aactacaata gtcatctatt
     1141 ctcatctttc tgagtccacc ccctgcaaag cccctcttta cgacataaaa attcggtcgg
     1201 aaaaggtatg caaaagatgt ttctctcttt aagagaaact cttcgggatg caaaaatatg
     1261 aaaataactc caattcacca aattatatag cgactttttt acaaaatgct aaaatttgtt
     1321 gatttccgtc aagcaattgt tgagcaaaaa tgtcttttac gataaaatga tacctcaata
     1381 tcaactgttt agcaaaacga tatttctctt aaagagagaa acaccttttt gttcaccaat
     1441 ccccgacttt taatcccgcg gccatgattg aaaaaggaag agtatgagta ttcaacattt
     1501 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1561 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1621 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1681 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1741 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1801 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1861 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1921 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1981 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2041 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2101 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2161 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tcacgcggta tcattgcagc
     2221 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2281 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2341 gtaactgtca gaccaagttt actcataacg cgtcaattcg agggggatca attccgtgat
     2401 aggtgggctg cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt
     2461 tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
     2521 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
     2581 gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc
     2641 tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca
     2701 gggttatgca gcggaaaacg gaattgatcc ggccacgatg cgtccggcgt agaggatctg
     2761 aagatcagca gttcaacctg ttgatagtac gtactaagct ctcatgtttc acgtactaag
     2821 ctctcatgtt taacgtacta agctctcatg tttaacgaac taaaccctca tggctaacgt
     2881 actaagctct catggctaac gtactaagct ctcatgtttc acgtactaag ctctcatgtt
     2941 tgaacaataa aattaatata aatcagcaac ttaaatagcc tctaaggttt taagttttat
     3001 aagaaaaaaa agaatatata aggcttttaa agcttttaag gtttaacggt tgtggacaac
     3061 aagccaggga tgtaacgcac tgagaagccc ttagagcctc tcaaagcaat tttgagtgac
     3121 acaggaacac ttaacggctg acatgggaat tcccctccac cgcggtgtat taatgcggct
     3181 gcaatttttt tgggcggggc cgcccaaaaa aatcctagca ccctgcaggg tctcccgctt
     3241 gatctggaag aagcaatgaa agctgctgtt aagtctccga atcaggtatt gttcctgaca
     3301 ggtgtattcc catccggtaa acgcggatac tttgcagttg atctgactca ggaataaatt
     3361 ataaattaag gtaagaagat tgtaggataa gctaatgaaa tagaaaaagg atgccgtcac
     3421 acaacttgtc ggcattcttt tttgttttat tagttgaaaa tatagtgaaa actttgcaac
     3481 agcatagctc agcacagatt atttgtcgta actttgcact ccagctgtca ccggatgtgc
     3541 tttccggtct gatgagtccg tgaggacgaa acagcctcta caaataattt tgtttaacgc
     3601 attttaaaat aaaataaatt atttatttaa ttaaacgaat atggttttta ctctggaaga
     3661 ttttgttggc gattggcgtc agaccgcggg ttataatttg gatcaagtcc tggaacaggg
     3721 tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg acgccgattc agcgcatcgt
     3781 tctgtccggc gagaacggtc tgaaaattga tattcatgtg atcatcccgt acgaaggcct
     3841 gagcggtgac caaatgggtc aaatcgagaa aatctttaaa gtcgtctacc cagttgacga
     3901 tcaccacttc aaggttatct tgcattacgg tacgctggtg attgatggtg tgaccccgaa
     3961 tatgattgac tatttcggcc gtccgtatga aggcattgcc gtttttgacg gtaaaaagat
     4021 caccgtcacc ggtaccctgt ggaatggcaa taagattatt gacgagcgtc tgattaaccc
     4081 ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc acgggttggc gtctgtgcga
     4141 gcgcatcctg gcataacaat gaatgggctt tcctcggtac caaattccag aaaagaggcc
     4201 tcccgaaagg ggggcctttt ttcgttttgg tccctaattg cctatcttcc agtgatggaa
     4261 cagcatttgt gcattggctg caacaatcag ccttacttgt gcctgttcta tttccgaacc
     4321 gaccgcttgt atgaatgcat caaaattcgt tttctctacg ttggattcct tgttgctcat
     4381 attgtgatga taatttctac aaatatagtc attggtaact atctatgaaa ctgtttgata
     4441 cttttatcag tccagtagtt ttacaaggtc tttcttcatt tcttcgtcaa tatccctgta
     4501 tcgtctgaaa gctttgcttc cctccttgtg tcccgacagt gcggaaacaa ggttcgggtc
     4561 tttcactttt ttatagatat tgccgataaa cgtacgtctt gccagatggc tgcttgccac
     4621 ttcataaata ggtcgtttga tttcgttgtg cgtcaacggg tctaagattg ttacgatgcg
     4681 gtcaactcca gctaatttga atatcttttt tatggcatca ttgtactttt gctcggatat
     4741 gaacggcaac agttttccct catattcttt gtagcgttca aggatttctt tcgctttgtc
     4801 gttaagtgga acacgtaccg taaccggatt cccctctttg gttttcttgg gaatatattc
     4861 tatggcttca ttgaccacat ttagtttggt cattcggtac aggtcgctca ccctgcatcc
     4921 tatcagtgtc tgaaatatga atatatccct ctgtattgcc agttgtgggg tggcagaaag
     4981 gtctgcatta aaaatcctgt ccctttcttc gagtgttata taataaggtg taccatatgt
     5041 acactcctct atcggaaact tgtcgaaagg tctgtttgtg gtgcgtttgt tatcgaagca
     5101 ccacaggaag aatgtgcgta ttcttgaaaa acagtctatc agcgtgtttt tgcttctggg
     5161 ctgtggtgtc ctcttttcgg gaatggcttc ataaatgctc gggtaaagtt cataatactg
     5221 gtattcgttc tgaaagaaat cccacatatc ccgaagcgtg tcaggtgtta ccaaatccac
     5281 atcaaggata aagccctttt gtcctctctt tgtagccctt acatatagtt cataacgcag
     5341 taaggctctt ttgacaaccc ggaaattttt ctttcgtact tccgacaaag ggtgcttgtt
     5401 tagaaattca tcgaatagtt ctccaatggt aggcttgata accacttcct ccggcaaaaa
     5461 atatttttca ggatggtaaa atttatcaag tgttgttttt agccattctt tgtctattgc
     5521 ttccttttct tgttgatata ccttctcgat ataggttttc aactggcgta tctcttcatt
     5581 tatatgggta cgcatttctt cattgcagac agctttcgtt tttacacatt cgtctttatc
     5641 atcccataga ttgggattga tggctaactg agtaggagca acggagtcta actgccttcc
     5701 gtttctaaaa cggacataga ttgtagccat ggattctgta tcatatcgtt tggctgcttt
     5761 tttaatgata aaggttactt tcatagactt tcaggttgaa ttttactctg ctgcaaatat
     5821 aaatattttc cccagcattt tccccacatc tgctaaatat tttgcaattc gattaaactt
     5881 ggattaaaat ttaataggat tataacatat tgaaatacag tgtaattgtg gcgtttttcc
     5941 gcatttttct ttttacccac attttcccca ctttaattat ttcaaatgcg gggtctgggt
     6001 acaagaaaga aagctaa
//
