LOCUS       sequence-31382-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP5
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    884..895
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggttcc gcgtggtagt atggcggagc tgagtgagga
      121 ggcgctgctg tcagtattac cgacgatccg ggtccctaag gctggagacc gggtccacaa
      181 agacgagtgc gccttctcct tcgacacgcc ggagtctgag gggggcctct acatctgtat
      241 gaacacgttt ctgggctttg ggaaacagta tgtggagaga catttcaata agaccggcca
      301 gcgagtctac ttgcacctcc ggcggacccg gcgcccgaaa gaggaggacc ctgctacagg
      361 cactggagac ccaccccgga agaagcccac gcggctggct attggtgttg aaggcggatt
      421 tgaccttagc gaggagaagt ttgaattaga cgaggatgtg aagattgtca ttttgccaga
      481 ttacctggag attgcccggg atggactggg gggactgcct gacattgtca gagatcgggt
      541 gaccagtgca gtggaggccc tactgtcggc cgactcagcc tcccgcaagc aggaggtgca
      601 ggcatgggat ggggaagtac ggcaggtgtc taagcatgcc ttcagcctca agcagttgga
      661 caaccctgct cgaatccctc cctgtggctg gaagtgctcc aagtgtgaca tgagagagaa
      721 cctgtggctc aacctgactg atggctccat cctctgtggg cgacgctact tcgatggcag
      781 tgggggcaac aaccacgctg tggagcacta ccgagagaca ggctacccgt tagctgtcaa
      841 gctgggcacc atcacccctg atggagctga cgtgtactca tatgatgagg atgacatggt
      901 cctggacccc agcctggctg a
//
