LOCUS       sequence-31383-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBQLN1
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             368..385
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(438..456)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      452..499
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      536..916
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(623..642)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     833..854
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag tcccaaaatc atgaaagtca
      121 ccgtgaagac cccgaaggaa aaggaggaat tcgccgtgcc cgagaatagc tccgtccagc
      181 agtttaagga agaaatctct aaacgtttta aatcacatac tgaccaactt gtgttgatat
      241 ttgctggaaa aattttgaaa gatcaagata ccttgagtca gcatggaatt catgatggac
      301 ttactgttca ccttgtcatt aaaacacaaa acaggccttg atgacgaagc ttgcggccgc
      361 actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc
      421 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      481 ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg
      541 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      601 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      661 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      721 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      781 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      841 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      901 attttgccga tttcggccta ttggttaaaa aatgactga
//
