LOCUS       sequence-31384-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP15
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             530..547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(600..618)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      614..661
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(785..804)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattccctct gaatattttg tttactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggttc cgcgtggtag tatggcggaa ggcggagcgg
      121 cggatctgga cacccagcgg tctgacatcg cgacgctgct caaaacctcg ctccggaaag
      181 gggacacctg gtacctagtc gatagtcgct ggttcaaaca gtggaaaaaa tatgttggct
      241 ttgacagttg ggacaaatac cagatgggag atcaaaatgt gtatcctgga cccattgata
      301 actctggact tctcaaagat ggtgatgccc agtcacttaa ggaacacctt attgatgaat
      361 tggattacat actgttgcca actgaaggtt ggaataaact tgtcagctgg tacacattga
      421 tggaaggtca agagccaata gcacgaaagg tggttgaaca gggtatgttt gtaaagcact
      481 gcaaagtaga agtatatctc tgatgacgaa gcttgcggcc gcactcgagc accaccacca
      541 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      601 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      661 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      721 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      781 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      841 gctctaaatc
//
