LOCUS       sequence-313844-       11775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian 
            cells for detection of tyrosinated microtubules..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Kesarwani S, Lama P, Chandra A, Reddy PP, Jijumon AS, Bodakuntla S, 
            Rao BM, Janke C, Das R, Sirajuddin M
  TITLE     Genetically encoded live-cell sensor for tyrosinated microtubules.
  JOURNAL   J Cell Biol. 2020 Oct 5;219(10). pii: 152071. doi: 
            10.1083/jcb.201912107.
  PUBMED    32886100
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-10-5"; volume: "219(10). pii: 152071. doi"; 
            pages: " 10.1083/jcb.201912107"
COMMENT     Sequence Label: TagRFP-T_A1aY1
FEATURES             Location/Qualifiers
     source          1..11775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          491..556
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             686..706
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1131..1265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1168..1187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1222..1241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1433..2066
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2113..2238
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2731..2964
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3149..3193
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3486..3603
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3652..4031
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4033..4308
                     /label=chicken beta-actin promoter
     intron          4309..5325
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5249..5271
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5333..5352
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      5390..5399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5396..6127
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     LTR             6627..6807
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8335..8354)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8568..8587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8687..8709
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8747..8765)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8833..8937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8938..9006,9007..9798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9156..9175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9969..10557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10458..10477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10711..10728
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10798..10818)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10803..11132
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10983..11118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
       61 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
      121 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
      181 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
      241 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
      301 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
      361 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
      421 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
      481 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
      541 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
      601 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
      661 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
      721 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
      781 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
      841 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
      901 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
      961 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     1021 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     1081 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     1141 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1201 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     1261 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     1321 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     1381 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     1441 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     1501 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     1561 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     1621 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     1681 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     1741 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     1801 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     1861 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     1921 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1981 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2041 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     2101 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     2161 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     2221 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     2281 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     2341 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     2401 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     2461 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     2521 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     2581 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     2641 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     2701 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     2761 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     2821 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     2881 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     2941 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3001 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3061 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3121 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3181 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3241 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     3301 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     3361 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     3421 cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc
     3481 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     3541 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     3601 tttcgggttt attacaggga cagcagagat ccactttggc tgatacgcgt tgacattgat
     3661 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     3721 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     3781 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     3841 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     3901 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     3961 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4021 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     4081 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     4141 gggggggggg ggggcgcgcc rggggggggg gggggggggg gggggggggg sggggcgagg
     4201 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     4261 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     4321 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     4381 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     4441 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     4501 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     4561 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     4621 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     4681 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     4741 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     4801 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     4861 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     4921 gccggggrgg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     4981 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     5041 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     5101 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     5161 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     5221 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     5281 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     5341 ctggttattg tgctgtctca tcattttggc aaagaattat tccgctagcg ccaccatggt
     5401 gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     5461 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     5521 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     5581 ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg gcatccccga
     5641 tttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     5701 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     5761 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
     5821 cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaac
     5881 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca agaccacata
     5941 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggaccacag
     6001 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt
     6061 ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaatggca tggacgagct
     6121 gtacaagtcc ggactcagat ctcgagtggc aacagtcaag ttcaaataca agggggagga
     6181 aaagcaggtg gacattagta agattctgag cgtcggaaga tacgggaagc tgatccactt
     6241 cctgtacgac ctgggaggag gcaaggcagg aatgggcatg gtgagcgaga aggatgcccc
     6301 caaggagctg ctccagatgc tggagaagca gaagaagtaa ggatccacta gtgtcgacct
     6361 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6421 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6481 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6541 gggggactgg aagggctaat tcactcccaa cgaagacaaa atcgtcgaga gatgctgcat
     6601 ataagcagct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     6661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6781 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6841 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattat aatagattta
     6901 gcaggaattg aactaggagt ggagcacaca ggcaaagctg cagaagtact tggaagaagc
     6961 caccagagat actcacgatt ctgcacatac ctggctaatc ccagatccta aggattacat
     7021 taagtttact aacatttata taatgattta tagtttaaag tataaactta tctaatttac
     7081 tattctgaca gatattaatt aatcctcaaa tatcataaga gatgattact attatcccca
     7141 tttaacacaa gaggaaactg agagggaaag atgttgaagt aattttccca caattacagc
     7201 atccgttagt tacgactcta tgatcttctg acacaaattc catttactcc tcaccctatg
     7261 actcagtcga atatatcaaa gttatggaca ttatgctaag taacaaatta cccttttata
     7321 tagtaaatac tgagtagatt gagagaagaa attgtttgca aacctgaata gcttcaagaa
     7381 gaagagaagt gaggataaga ataacagttg tcatttaaca agttttaaca agtaacttgg
     7441 ttagaaaggg attcaaatgc ataaagcaag ggataaattt ttctggcaac aagactatac
     7501 aatataacct taaatatgac ttcaaataat tgttggaact tgataaaact aattaaatat
     7561 tattgaagat tatcaatatt ataaatgtaa tttactttta aaaagggaac atagaaatgt
     7621 gtatcattag agtagaaaac aatccttatt atcacaattt gtcaaaacaa gtttgttatt
     7681 aacacaagta gaatactgca ttcaattaag ttgactgcag attttgtgtt ttgttaaaat
     7741 tagaaagaga taacaacaat ttgaattatt gaaagtaaca tgtaaatagt tctacatacg
     7801 ttcttttgac atcttgttca atcattgatc gaagttcttt atcttggaag aatttgttcc
     7861 aaagactctg aaataaggaa aacaatctat tatatagtct cacacctttg ttttactttt
     7921 agtgatttca atttaataat gtaaatggtt aaaatttatt cttctctgag atcatttcac
     7981 attgcagata gaaaacctga gactggggta atttttatta aaatctaatt taatctcaga
     8041 aacacatctt tattctaaca tcaatttttc cagtttgata ttatcatata aagtcagcct
     8101 tcctcatctg caggttccac aacaaaaatc caaccaactg tggatcaaaa atattgggaa
     8161 aaaattaaaa atagcaatac aacaataaaa aaatacaaat cagaaaaaca gcacagtata
     8221 acaactttat ttagcattta caatctatta ggtattataa gtaatctagc cagatcctct
     8281 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
     8341 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
     8401 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
     8461 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
     8521 taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct
     8581 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8641 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8701 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     8761 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8821 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8881 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8941 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9001 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9061 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9121 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9181 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9241 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9301 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9361 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9421 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9481 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9541 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9601 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9661 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9721 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9781 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9841 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9901 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9961 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10021 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    10081 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    10141 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10201 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    10261 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    10321 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10381 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10441 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10501 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10561 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10621 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10681 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    10741 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga
    10801 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10861 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
    10921 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
    10981 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    11041 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    11101 gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag
    11161 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
    11221 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
    11281 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
    11341 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
    11401 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg
    11461 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
    11521 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
    11581 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
    11641 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
    11701 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
    11761 tattcgtccc gccaa
//
