LOCUS       sequence-313848-        8035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of GCaMP6f in a piggybac plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8035)
  TITLE     Lian Lab PB plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB-UBC-GCAMP6f-PGK-neo
FEATURES             Location/Qualifiers
     source          1..8035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        149..1360
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1197..1216
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1375..1384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1381..1389,1390..1407,1408..1410,1411..1443,1444..1446,
                     1447..1470,1471..1497,1498..1554,1555..1560,1561..1830,
                     1831..1848,1849..2283,2284..2289,2290..2733)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      1477..1486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1767..1788
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1894..1915)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2155..2174)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2758..2806
                     /note="synthetic polyadenylation signal"
     protein_bind    2852..2885
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2950..3449
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2985..3008)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3382..3401
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        3461..3508
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3527..4330
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3590..3609)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4200..4219
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4362..4379)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4368..4592
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4649..4682
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   4879..4941
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4992..5012)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4992..5010)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5031..5047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5031..5047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5044..5066)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5055..5071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5079..5085,5086..5103,5104..5109))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5124..5145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5262..5279)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5433..6021)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5513..5532)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6192..6983,6984..7052))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6815..6834
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7053..7157)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7183..7638)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7320..7341)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7532..7551
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7765..7787
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7779..7796
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7780..7796
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7806..7825
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7806..7824
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   7854..7888
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
       61 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
      121 agatatcaac tagaatgcta gcctcgaggg cctccgcgcc gggttttggc gcctcccgcg
      181 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
      241 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
      301 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
      361 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
      421 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
      481 ctagttccgt cgcagccggg atttgggtcg cagttcttgt ttgtggatcg ctgtgatcgt
      541 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
      601 gtgggacgga ggcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
      661 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
      721 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
      781 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
      841 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
      901 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
      961 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     1021 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     1081 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     1141 ataagtgagg cgtcagtttc tctggtcggt tttatgtacc tatcttctta agtagctgaa
     1201 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     1261 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     1321 gtccgctaaa ttctggccgt ttttggcttt tttgttagac ggatccatga attcgccacc
     1381 atgggttctc atcaccatca tcatcatggt atggctagca tgactggtgg acagcaaatg
     1441 ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt
     1501 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag
     1561 aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc
     1621 cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
     1681 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg
     1741 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1801 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag
     1861 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1921 ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc
     1981 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2041 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2101 ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac
     2161 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2221 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2281 aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagagga attctcccta
     2341 tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct
     2401 ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac
     2461 ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac
     2521 agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc
     2581 tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat
     2641 gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac
     2701 gaagagtttg tacaaatgat gacagcgaag taatgatcta gaaagcttca tggccgcaat
     2761 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgtcaa ttcctgcagc
     2821 ccaattccga tcatattcaa taacccttaa tataacttcg tataatgtat gctatacgaa
     2881 gttattaggt ctgaagagga gtttacgtcc agccaagcta gcttggctgc aggtcgtcga
     2941 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     3001 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     3061 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     3121 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     3181 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     3241 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     3301 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     3361 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     3421 tctcctcttc ctcatctccg ggcctttcga cctgcagcct gttgacaatt aatcatcggc
     3481 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg gatcggccat
     3541 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3601 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3661 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     3721 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     3781 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     3841 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     3901 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     3961 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4021 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4081 tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4141 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4201 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4261 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4321 gttcttctga ggggatcaat tctctagagc tcgctgatca gcctcgactg tgccttctag
     4381 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4441 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4501 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4561 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4621 ctcgactaga gcttgcggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt
     4681 attaggtccc tcgacatact agttaaaagt tttgttactt tatagaagaa attttgagtt
     4741 tttgtttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
     4801 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
     4861 atacagaccg ataaaacaca tgcgtcaatt ttacgcatga ttatctttaa cgtacgtcac
     4921 aatatgatta tctttctagg gttaatctag tatacgcgta tgcggccgct taattaatcc
     4981 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     5041 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5101 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5161 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5221 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5281 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5341 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5401 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6001 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6061 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6121 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6181 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6241 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6301 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6361 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6421 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6481 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6541 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6601 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6661 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6721 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6781 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6841 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6901 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6961 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7021 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7141 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     7201 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     7261 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     7321 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     7381 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     7441 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     7501 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     7561 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     7621 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     7681 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     7741 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     7801 gcgcgtaata cgactcacta tagggcgaat tggggcgcgc cattctagat taaccctaga
     7861 aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct ctttctaaat
     7921 agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag ctcccgtgag
     7981 gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg cgtga
//
