LOCUS       sequence-313851-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3510
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtagat gtaacctcag tatctatagt
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tgggcagtcc cactccccct ccccctcagc gtcgtcctcg
      241 tgcggtgcct ccaggtcgtt gaggtggaag ttggccatca tgtcgcgcac agccagcatc
      301 agcctgttca ggccctggtt gcggttcaga cccccagcca ctccttcctc gggcctctcc
      361 ccctccatgg tatagtttgg caacagtgac cggaagaaga gagcaatggt gtttccatgg
      421 ctgataggac ttagcctctc tggcctgacg taggagtaga ttgtgtccga aggaggcaga
      481 ggatcaaacc ccatcacaga ctgcgtggtc acgtccgggg gcagggcagc gacggcttcc
      541 ttgatctcag agaggatcac atggcggtgg atattcctgg gtgcacgctg gtagagcacc
      601 ttccgccggt tctcacaggc ttccacggct gggtccccgg cgtccactgc ttgcagaacc
      661 tcgtggacgt tctcctccag ccagctcatg gtggcgggct ctttccagag aaagtgtgac
      721 ctcc
//
