LOCUS       sequence-313859-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3517
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(486..510)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             518..559
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tttggggggc ggggcggcgg cgcccgagga cgacgatgaa gacgacgacg aggagctgct
       61 ggaagtgcgc ctggtcgaga ccccggggcg ggagctgtgg aggatggtcc cggcgggacg
      121 ggccgctcgg ggacaagcgg agcgcgccca agggccgtcg ggcgaggggg cggccgccgc
      181 cgccgccgcc tccccgacac cgtcggagga cgaggagccg gaggaagagg aggaggaggc
      241 ggcagcggct gaggaaggtg aagaggagac ggtggcgtcg ggggaggagt cgctgggctt
      301 tctgtccagg ctgccccctg gcccggccgg cctggactgc agcgccctgg atcgcgaccc
      361 ggacctgcag cctccctcgg gcacgtcgca cttcgagttc ccggactact gcacccccga
      421 ggttaccgag atgatcgcgg gggactggcg cccgtctagc atcgcagacc tggttttcac
      481 ctacttgcca actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct
      541 cctcggtctc gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
      601 ttggctttat atatcttgtg gaaaggacga ataagtggcg gtgattaatt acaaacgcgt
      661 taagtcgaca atcaacctct ggattacaaa att
//
