LOCUS       sequence-31386-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HK3
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atattttgtt tactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtc ggcgcctgct ggaggagacc ctggccccat
      121 tccggttgaa ccatgatcaa ctggctgcgg ttcaggcaca gatgcggaag gccatggcca
      181 aggggctccg aggggaggcc tcctcccttc gcatgctgcc cactttcgtc cgggccaccc
      241 ctgatggcag cgagcgaggg gatttcctgg ccctggacct cgggggcacg aacttccgtg
      301 tcctcctggt acgtgtgacc acaggcgtgc agatcaccag cgagatctac tccattcccg
      361 agactgtggc ccagggttct gggcagcagc tctttgacca catcgtggac tgcatcgtgg
      421 acttccagca gaagcagggc ctgagcgggc agagcctccc actgggtttt accttctcct
      481 tcccatgtag gcagcttggc ctagaccagg gcatcctcct gaactggacc aagggtttca
      541 aggcatcaga ctgcgagggc caagatgtcg tgagtctgtt gcgggaagcc atcactcgca
      601 gacaggcagt ggagctgaat gtggttgcca ttgtcaatga cacggtgggg accatgatgt
      661 cctgtggcta tgaggacccc cgttgcgaga taggcctcat tgtcggaacc ggcaccaatg
      721 cctgctacat ggaggagctc cggaatgtgg cgggcgtgcc tggggactca ggccgcatgt
      781 gcatcaacat ggagtggggc gcctttgggg acgatggctc tctggccatg ctcagcaccc
      841 gctttgatgc aagtgtggac caggcgtcca tcaaccccgg caagcagagg tttgaaaaga
      901 tgatcagcgg catgtacct
//
