LOCUS       sequence-313860-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3519
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtggta ctcctagcgg agtaggggtc
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagc tgggtcttac atctgtccca tgtgattcga tgcgtctccc
      241 attccagggt gtgggccggc caaggagaga gggggaggct ctgaggacac cttctcttcc
      301 tcccttcttt tcagacacgc agctttcgga ttcagattcc tttctcggac ttgctgctcc
      361 agactgagga tgacggccac cgcctggtgg aggatcagga gcttggtctg gggcttgtca
      421 ctcttgaggt ggagctgcac catgcggccg agctctttga aagcctcgtt gatgtcacgg
      481 acccgcagac gctctcgggc attgttggcc atcctccgct ccttctcacg ctctgccttc
      541 tgctctggtg tcaggtcctc atcgtcatta ttgctagtaa ttgatttgat atccttcttg
      601 tcgtcatcta atttcttgtc ctccgaagat ttcgtgtctt gcaggttctc atcaccctcg
      661 tcatcggatt tgatctcaga gctgccagag gagacactct gcccctgtag tcctggtg
//
