LOCUS       sequence-313862-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3520
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtaggt tgcctagaag actccgagtc
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tggcaacatc tgtcccatgt gattcgatgc gtctcccatt
      241 ccagggtgtg ggccggccaa ggagagaggg ggaggctccg aggacacctt ctcttcctcc
      301 cttcttttca gacacgcagc tttcggattc agattccttt ctcggacttg ctgctccaga
      361 ctgaggatga cggccaccgc ctggtggagg atcaggagct tggtctgggg cttgtcactc
      421 ttgaggtgga gctgcaccat gcggccgagc tctttgaaag cctcgttgat gtcacggacc
      481 cgcagacgct ctcgggcatt gttggccatc ctccgctcct tctcacgctc tgccttctgc
      541 tctggtgtca ggtcctcatc gtcattattg ctagatcttg acctagtaat tgatttgata
      601 tccttcttgt cgtcatctaa tttcttgtcc tccgaagatt tcgtgtcttg caggttctca
      661 tcaccctcgt catcggattt gatctcagag ctgccagagg agacactctg cccctgtagt
      721 cctggtgg
//
