LOCUS       sequence-313863-         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3540
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(417..441)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             449..490
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcatcgattg
       61 atcaacaagt ttgtacaaaa aagttggcac catgaccagg aagatcttca caaataccag
      121 ggagcggtgg aggcagcaga atgtcaacag cgcctttgcc aagctgagga agctcatccc
      181 cactcaccct ccagacaaaa agctgagcaa aaatgaaacg cttcgcctgg caatgaggta
      241 tatcaacttc ttggtcaagg tcttggggga gcaaagcctg caacaaacgg gagtggctgc
      301 tcaggggaac attctggggc tcttccctca aggaccccac ctgccaggcc tggaggacag
      361 aactctgctt gagaactacc aggttccttc acctggtcca agccaccaca ttcctttgcc
      421 aactttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
      481 cgattctacg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
      541 tatatcttgt ggaaaggacg aaggatcctg taccctagtg acaacacgcg ttaagtcgac
      601 aatcaacctc tggattacaa aat
//
