LOCUS       sequence-313864-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3521
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtctta aagaaaagga taagatatct
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tgggtatgca ctggggatcc gagtgtccag gagctccggc
      241 ttggcatcca ctgtggcagg tgcagggtcc tgggcaagga ccatggctat gtcaggccct
      301 ccacagatga tggtgaaggt ttgggattcc tcaggtccaa gggtctgggc tgttgtcagg
      361 cgctgcagac cccggcgtgc ccgtgccagc aggctctccc cgtgtagctg ctgtgccagc
      421 cgttccaggg cctgcagctg cgcctggtgc cgctcctggc accgttgcag cctccgcacc
      481 cggcgttgca gtgctcccag cactgggccc agcccggtct gggcctgttg ggcagggact
      541 tcaggttgtg accgctcagg agttggcgca ggggccaggg gggtcaggag catggtggcc
      601 acagtcttgg ggctccccga tgtggggccc agcaccacta ggcgcactgg gccagagact
      661 gggatggcag ggctctgggg caggggtgta ttcttctgta ggggaggcgg cggcgagact
      721 ggcttctggg tgcttcgggt cctccgctga ctcttggcag gtggtccccg ggagaagatg
      781 gagg
//
