LOCUS       sequence-313867-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3522
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtctgt ggttctcaac taacagtgac
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tggcaaaggg atctgaaaga cgttcagctt gctggtgttc
      241 ttgagcgtct ggcgccgatt gcagaaccag acccgcacta cctcacggtc gtagttgagc
      301 tccttagcaa tttcagtgat ctcctggcct gtgggcagtg ggttcttctc aaaataggca
      361 ttgagagcct ctatggcctg gggggtgaag gaggtgcggc gtttgcgttt cttggagggc
      421 tcgcctccca caaactccat caggttctgc tggccttcct ggttccgcag ttcagcttcg
      481 tttagccact tttccagcac cggctttagc ttctgggcac tcttgggtgt gatgtctagc
      541 ttctcgaacc ggcagatggc tgactggctg taggctggac cttccgttgc agtcagagcc
      601 tgacccacct gggtctgtgt aaggcccagc gagagccgcc ggatcttaaa gttcttggca
      661 aactcccgga tctcttctaa gttgatccca tcctcatcca gacttggagt atgtggcttg
      721 g
//
