LOCUS       sequence-313868-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3528
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..33
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(493..517)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             525..566
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 attgatcaac aagtttgtac aaaaaagttg gcaccatggc caacctggag cgcaccttca
       61 tcgccatcaa gccggacggc gtgcagcgcg gcctggtggg cgagatcatc aagcgcttcg
      121 agcagaaggg attccgcctc gtggccatga agttcctccg ggcctctgaa gaacacctga
      181 agcagcacta cattgacctg aaagaccgac cattcttccc tgggctggtg aagtacatga
      241 actcagggcc ggttgtggcc atggtctggg aggggctgaa cgtggtgaag acaggccgag
      301 tgatgcttgg ggagaccaat ccagcagatt caaagccagg caccattcgt ggggacttct
      361 gcattcaggt tggcaggaac atcattcatg gcagtgattc agtaaaaagt gctgaaaaag
      421 aaatcagcct atggtttaag cctgaagaac tggttgacta caagtcttgt gctcatgact
      481 gggtctatga attgccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta
      541 accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt
      601 cgatttcttg gctttatata tcttgtggaa aggacgacca aggcatcgcg tctcaaagta
      661 aacgcgttaa gtcgacaatc aacctctgga ttacaaaat
//
