LOCUS       sequence-313869-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3523
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtgccg tgcggtgttc gccctcggat
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tgggtacagg cagtattgct gcttgttgtg tacacgctga
      241 tggcggctga ggctcgagtg gcaccgaaag gctttctggc aacgttgaca ctgataaagt
      301 gtctcctggg tgtgaactct caggtgctgg ttgagatggg cactgaggac aaaagctttg
      361 ccacacacat tacaggagta aggcttctcc cctgtgtgaa ttctctggtg ctgtgttaga
      421 tttgctttct gggcaaaggc tttcccacat tcaccacatc tgtagggctt ctccccagtg
      481 tgaactctcc ggtgaataga aaggcatgag ctctgggtga aggctttccc gcattcctga
      541 caaacataag gcttttctcc agtgtgaatt ctctggtggc ggacaagctc agcactcagg
      601 ccgaaggcct tcccgcagta atcacataca tagggcttct cacctgagtg aattctctga
      661 tgcttagaaa ggcatgagct ctgacggaac gttttcccgc attcgttaca caccaacggc
      721 ttctttctgg catgagttc
//
