LOCUS       sequence-313872-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3516
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(518..542)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             550..591
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gaccttgaaa atataatgag gaagttttct ggtagctgca gatgctgtgc aaaacagatt
       61 aaattctatc gcatgagaca tcattacaaa tcttgtaaga agtatcagga tgaatatggt
      121 gtttcttcta tcattccaaa ctttcagatc tctcaagatt cagtagggaa cagcaatagg
      181 agtgaaacat ccacatctga taacacagaa acttaccaag agaatacaag ttcttctggt
      241 catcctactt ttaagtgtcc cctgtgtcaa gaatcaaatt ttaccagaca gcgtttactg
      301 gatcactgta acagtaatca cctatttcag atagttcctg tgacatgtcc tatttgtgtg
      361 tctcttcctt ggggagatcc tagccagatt accagaaatt tcgttagtca tctaaatcag
      421 agacatcaat ttgattatgg agaatttgtg aatcttcagc tagatgaaga aacccaatac
      481 caaactgctg ttgaagaatc ttttcaagta aacatctgcc caactttctt gtacaaagtg
      541 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
      601 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      661 gaaacggcgg tgctaggcgg ggcccaacgc gttaagtcga caatcaacct ctggattaca
      721 aaat
//
