LOCUS       sequence-313875-         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3532
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtctgc aggatttcga atgacgtatc
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tggcaatgtc tttttccttt tttgaactga aggccccttt
      241 ttgctttgct cttctggtgc tatagatgac ttcgtggagg aagcttcttg aggtgacctg
      301 cctgaccgtt ctgagatctc tgccctagcc tcctgaggaa acggtggtga tgtacagcca
      361 tgcccgcctc ccacttctcg agggagagtt gaagtgcatg gctgggattc tgcttcctgt
      421 cttccctgag aactcgcggg tggctgtgca acatttttat gtgggcagtt tgccaccatg
      481 tgcatgatgc tctgacagta atggcacttc tttggctgag gaggtagact acattcctta
      541 gcatgatgat caaggccacc acagttgtag catctatctc cctttggttt tcttttctgt
      601 agtgtcttcc ctttgggtct tctttcactt cctaaacagg ggctcccacc aggtcctgtt
      661 acccgtattg actcaaggc
//
