LOCUS       sequence-313877-         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3527
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(368..392)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             400..441
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gtggagccca tctcggaagg cgagtcggag gaggtagagg tgactgacat cagtgatgag
       61 gatgaggaag acggggaggt gttcaagacg ccccgtgccc cacctgcacc ccctaagcct
      121 gagcccggcg aggcacccgg ggcatcccag tgcatgcccc tcaagctacg ctttaagcgg
      181 cgctggagtg aagactgtcg cctcgaaggg ggtgggggcc ccgctggggg ctttgaggat
      241 gagggtgagg acaagaaggt gcgtggggag gggcctgggg aggctggggg gcccctcacc
      301 ccaaggcggg tgagctctga cctccagcat gccacggccc agctctccct ggagcaccga
      361 gactcctgcc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
      421 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
      481 tcttggcttt atatatcttg tggaaaggac gaccagtcct cggcgcacga ataggtacgc
      541 gttaagtcga caatcaacct ctggattaca aaa
//
