LOCUS       sequence-313878-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3533
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtgcag caaacgatct cggccaagac
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tggctagcac aggcgcttgt acgtgtagat gtaggctttg
      241 cagctggggc atgtgtgcgt cacatccttg aagtcattga tgaggcaggg gatcaggcag
      301 cagcccagat cacatcccat gaagcaacag aagaaaccca gcacgaaatt catcaagcca
      361 atctcgtagg agatcttggt ggtgatggcc tgctggcagt ggggacacac cgtctgcaca
      421 ggcgctccct caaagatctc tccctgcagc actgtcaccg tggtggcagc tcctgaaggg
      481 accaggactg tggctgtgtg gcccccaggg ccagggtagg gccctggcgt gtagggccct
      541 ggggggtagt agcccatggg tgggtggggg cctggaggag ggtagaaacc cggaggcatg
      601 taggtgccat ctgcactcat gtgtggtggg atgaagccag gctggggcat tgggtgaccc
      661 ggcggctcat agggtggggg
//
