LOCUS       sequence-313882-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3534
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtctta gcagcgtgtg gcggaacatc
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tggcaactgt tgggtcttct ctgaagaggc ctggagggag
      241 gtggggtccc cacgtgccct cgccgtacct ggcgagcgct ctgggctggt gccaccgggt
      301 gatctcctac cggggccagc cgtcggccaa gggcagccgg cgcagggcgg gcccaggccg
      361 cagaggctct gccagggacc gggcgaggag caagggatag gggaaggtct taggaaagtc
      421 gccggggccc ccgggggccg gagagaggcg ctcagacttg atgctgactg ggggggtcgg
      481 cggggaggcg ccgcgggttg ggggaccctc ctcgcccagg cttcggcccc cactgggggg
      541 cctccgggga ggaaggggaa gctccccagt gggggtccgg gagttgcagt ggagcagggg
      601 ttctgcaggc cactgtagag gcttctggag gtgtttagtc cccctcgggg cccagccagg
      661 ccaccaggca ggtctgacct ccgcccttcc gtcggcaggt acagtggggg tggtgtcttg
      721 ctggtga
//
