LOCUS       sequence-313883-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3538
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(534..558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             566..607
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ccaagagtac aataatagcc ccgaaggcag ctccactgtc atgctccagc tcaaccctcg
       61 tggcgcggcc aagccgcagc tctccgctgc ccagctgcag atggaaaaga agatgaacga
      121 gcccgtgagc ggccaggagc ccaccaaagt ctcccaggtg gagagccccg aggccaaagg
      181 cggccttccc gaagagagga gctgcctggc tgaggtctcc gtgtccagtc ccgaagtgca
      241 ggagaaggaa agcaaagagg aaatcaagtc tgatacacca accagcaatt ggctcactgc
      301 aaagagtggc agaaagaaga ggtgccctta cactaagcac caaacgctgg aattagaaaa
      361 agagttcttg ttcaatatgt acctcacccg cgagcgccgc ctagagatca gtaagagcgt
      421 taacctcacc gacaggcagg tcaagatttg gtttcaaaac cgccgaatga aactcaagaa
      481 gatgagccga gagaaccgga tccgagaact gaccgccaac ctcacgtttt cttacccaac
      541 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
      601 ttctacgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
      661 atcttgtgga aaggacgaca actcatgcgg ccacgattca gcacgcgtta agtcgacaat
      721 caacctctgg attacaaaat
//
