LOCUS       sequence-313886-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3536
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..173)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    181..205
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttgtaatcc agaggttgat tgtcgactta acgcgtaaaa tagcttatat agcagtaccg
       61 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggacta
      121 gttcattact acgtagaatc gagaccgagg agagggttag ggataggctt accgatatca
      181 accactttgt acaagaaagt tgggtataag gtcgtcaggt ccgtgtggtg gtctttggcc
      241 atcggctgta aatgctggct gggggcgact gaggaggagc aagatgagga ggaagatgag
      301 gacgatgacc tgcctttagt gttgggcagc ttatgatctt ttttccactt catcctccgg
      361 ttctggaacc agatcttgat ctggcgctcc gacagacaca gggtgtgagc gatttcaatc
      421 cgacggcgcc ttgtcagata cctgttaaaa tgaaattctt tttccagttc taggacttgc
      481 tgccgggtgt aggccgttcg ggaccgcttg ggttccccac cggtgtagtt ggggttcacc
      541 gaattcacgt gcaccttctt catccagggg tagaccacgg ccggctgctt gagtgccgtc
      601 ccggagggcg gctgcttggg gtcggactga ctgtaggccc gggcgccagg caggggcgcc
      661 ggcggaggcg ccgggggagc tgggcaaggc tctcctggag cggcgtagtg accgccgggg
      721 ccgcctggct cttgtccgtg accccgcgca ggcagcgccg a
//
