LOCUS       sequence-313903-        9554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP38
FEATURES             Location/Qualifiers
     source          1..9554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             439..462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             475..501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    517..541
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3668..3692)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3705..4272
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3722..4274
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3866..3883)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4093..4112
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4286..4885
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4286..4305)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4782..4802
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4961..5475
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5637..5653
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5661..5667,5668..5685,5686..5691))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5706..5727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5844..5861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6015..6603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6095..6114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6774..7565,7566..7634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7397..7416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7635..7739)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7807..7825
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7863..7885)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7985..8004
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8198..8220
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8348..8367
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             8567..9083
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9147..9488
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
      421 attagatctg ccaccatgga ctacaaggat gacgatgaca agctcgatgg aggataccca
      481 tacgatgttc cagattacgc tgctcgaggt tatcaaacaa gtttgtacaa aaaagttggc
      541 atggacaaga tcctggaggg ccttgtgagt tcctcgcatc ccctgcccct caagcgggtg
      601 attgtgcgga aggtggtgga atcggcggag cactggctag acgaggcgca gtgcgaggcc
      661 atgtttgacc tgacgacccg gctcatcctg gagggccagg accctttcca gcggcaggtg
      721 gggcaccagg tgctggaggc ctacgcacga taccaccggc cagagttcga gtccttcttc
      781 aacaagacct tcgtgttggg cctccttcat cagggctacc actctctgga caggaaggat
      841 gtagccatcc tggactacat tcacaacggc ctgaagctga ttatgagctg tccgtcggtg
      901 ctggatctct ttagcctcct gcaggtagag gtgttacgga tggtgtgtga gaggccggag
      961 ccgcagctct gtgcccgact gagcgacctt ctgaccgact ttgtgcaatg catccccaag
     1021 gggaaattgt ccatcacgtt ctgtcaacag ctggttcgaa cgataggcca tttccagtgc
     1081 gtgtccaccc aggaaagaga gctgcgggaa tatgtctccc aggtgacaaa agtgagtaac
     1141 ttgctgcaga acatctggaa ggccgagcct gccacactac tgccttccct gcaagaagtt
     1201 tttgcaagca tctcttccac agatgcatca tttgaacctt ctgtagcatt ggcaagcctt
     1261 gtgcagcata ttcctcttca gatgattaca gttctcatca ggagccttac tacggatcca
     1321 aatgtaaaag atgcaagtat gacccaagcc ctttgcagaa tgattgactg gctatcctgg
     1381 ccattggctc agcatgtgga tacatgggta attgcactcc tgaaaggact ggcagctgtc
     1441 cagaagttta ctattttgat agatgttact ttgctgaaaa tagaactggt ttttaatcga
     1501 ctttggtttc ctcttgtgag acctggtgct cttgcagttc tttctcacat gctgcttagc
     1561 tttcagcatt ctccagaggc gttccatttg attgttcctc atgtggttaa tttggttcat
     1621 tctttcaaaa atgatggtct gccttcaagt acagccttct tagtacaatt aacagaattg
     1681 atacactgta tgatgtatca ttattctgga tttccagatc tctatgaacc tattctggag
     1741 gcaataaagg attttcctaa gcccagtgaa gagaagatta agttaattct caatcaaagt
     1801 gcctggactt ctcaatccaa ttctttggcg tcttgcttgt ctagactttc tggaaaatct
     1861 gaaactggga aaactggtct tattaaccta ggaaatacat gttatatgaa cagtgttata
     1921 caagccttgt ttatggccac agatttcagg agacaagtat tatctttaaa tctaaacggg
     1981 tgcaattcat taatgaaaaa attacagcat ctttttgcct ttctggccca tacacagagg
     2041 gaagcatacg cacctcggat attctttgag gcttccagac ctccatggtt tactcccaga
     2101 tcacagcaag actgttctga atacctcaga tttctccttg acaggctcca tgaagaagaa
     2161 aagatcttga aagttcaggc ctcacacaag ccttctgaaa ttctggaatg cagtgaaact
     2221 tctttacagg aagtagctag taaagcagca gtactaacag agacccctcg tacaagtgac
     2281 ggtgagaaga ctttaataga aaaaatgttt ggaggaaaac tacgaactca catacgttgt
     2341 ttgaactgca ggagtacctc acaaaaagtg gaagccttta cagatctttc gcttgccttt
     2401 tgtccttcct cttctttgga aaacatgtct gtccaagatc cagcatcatc acccagtata
     2461 caagatggtg gtctaatgca agcctctgta cccggtcctt cagaagaacc agtagtttat
     2521 aatccaacaa cagctgcctt catctgtgac tcacttgtga atgaaaaaac cataggcagt
     2581 cctcctaatg agttttactg ttctgaaaac acttctgtcc ctaacgaatc taacaagatt
     2641 cttgttaata aagatgtacc tcagaaacca ggaggtgaaa ccacaccttc agtaactgac
     2701 ttactaaatt attttttggc tccagagatt cttactggtg ataaccaata ttattgtgaa
     2761 aactgtgcct ctctgcaaaa tgctgagaaa actatgcaaa tcacggagga acctgaatac
     2821 cttattctta ctctcctgag attttcatat gatcagaagt atcatgtgag aaggaaaatt
     2881 ttagacaatg tatcactgcc actggttttg gagttgccag ttaaaagaat tacttctttc
     2941 tcttcattgt cagaaagttg gtctgtagat gttgacttca ctgatcttag tgagaacctt
     3001 gctaaaaaat taaagccttc agggactgat gaagcttcct gcacaaaatt ggtgccctat
     3061 ctattaagtt ccgttgtggt tcactctggt atatcctctg aaagtgggca ttactattct
     3121 tatgccagaa atatcacaag tacagactct tcatatcaga tgtaccacca gtctgaggct
     3181 ctggcattag catcctccca gagtcattta ctagggagag atagtcccag tgcagttttt
     3241 gaacaggatt tggaaaataa ggaaatgtca aaagaatggt ttttatttaa tgacagtaga
     3301 gtgacattta cttcatttca gtcagtccag aaaattacga gcaggtttcc aaaggacaca
     3361 gcttatgtgc ttttgtataa aaaacagcat agtactaatg gtttaagtgg taataaccca
     3421 accagtggac tctggataaa tggagaccca cctctacaga aagaacttat ggatgctata
     3481 acaaaagaca ataaactata tttacaggaa caagagttga atgctcgagc ccgggccctc
     3541 caagctgcat ctgcttcatg ttcatttcgg cccaatggat ttgatgacaa cgacccacca
     3601 ggaagctgtg gaccaactgg tggagggggt ggaggaggat ttaatacagt tggcagactc
     3661 gtattttgcc caactttctt gtacaaagtg gttcgataac gaattccgcc ccccccccct
     3721 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3781 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3841 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3901 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3961 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     4021 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     4081 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     4141 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     4201 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     4261 acacgatgat aatatggcca caaccatgac cgagtacaag cccacggtgc gcctcgccac
     4321 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4381 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4441 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4501 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4561 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     4621 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     4681 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4741 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4801 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4861 catgacccgc aagcccggtg cctgatgcat cgatgtcgac ctgcagccaa gcttatcgat
     4921 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     4981 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga
     5041 atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga
     5101 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     5161 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     5221 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     5281 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
     5341 ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     5401 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     5461 gcgggggtct ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg
     5521 agtcgaatat taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat
     5581 actcctgaaa tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt
     5641 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5701 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5761 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     5821 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7741 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     7801 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     7861 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7921 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     7981 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8041 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     8101 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     8161 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     8221 acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     8281 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     8341 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     8401 cggccacgat gcgtccggcg tagaggcgat tagtccaatt tgttaaagac aggatatcag
     8461 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc
     8521 catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
     8581 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata
     8641 actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca
     8701 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     8761 cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     8821 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     8881 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
     8941 gccagtcctc cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct
     9001 gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga ttgattgact
     9061 gcccacctcg ggggtctttc atttggaggt tccaccgaga tttggagacc cctgcccagg
     9121 gaccaccgac ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg tctgtctctg
     9181 tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac tagttagcta
     9241 actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca cccggccgca
     9301 accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc tgaggaaggg
     9361 agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac gagaacctaa
     9421 aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt
     9481 gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt
     9541 tgtctgaaaa ttag
//
