LOCUS       sequence-313925-       10021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP52
FEATURES             Location/Qualifiers
     source          1..10021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      430..439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             439..462
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             475..501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    517..541
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4135..4159)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4172..4739
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4189..4741
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4333..4350)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4560..4579
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4753..5352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4753..4772)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5249..5269
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5428..5942
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6104..6120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6128..6134,6135..6152,6153..6158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6173..6194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6311..6328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6482..7070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6562..6581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7241..8032,8033..8101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7864..7883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8102..8206)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8274..8292
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8330..8352)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8452..8471
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8665..8687
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8815..8834
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             9034..9550
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9614..9955
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
      421 attagatctg ccaccatgga ctacaaggat gacgatgaca agctcgatgg aggataccca
      481 tacgatgttc cagattacgc tgctcgaggt tatcaaacaa gtttgtacaa aaaagttggc
      541 atgaactttg agggtctgga ccctggactg gcagaatatg ccccagccat gcattctgcc
      601 ctggaccctg tcttggatgc ccacctgaac ccaaatctgc tacagaatgt ggagctggac
      661 ccagagggag tggccttgga ggctcttccc gtccaggaat cagtgcacat aatggaaggt
      721 gtctactctg aattgcacag cgtggtggct gaagtgggtg tacctgtttc cgtctcccac
      781 tttgacttgc acgaggagat gctgtgggtg gggagccacg ggggccatgc cacttcattt
      841 tttggcccag ccttggagcg ctactcatcc tttcaagtca atggcagtga tgatattcgg
      901 cagatccaga gcctggagaa tggtatcctt tttctcacca agaacaacct caagtatatg
      961 gcccgtgggg gcctcattat atttgattac ctgctggatg agaatgagga tatgcacagt
     1021 ctcctactga ctgacagcag cactctactc gttggtgggc tgcagaatca catactagag
     1081 attgatctta acactgtcca ggagactcag aagtatgcag tagagacgcc tggagtcacc
     1141 atcatgagac agacaaatcg cttcttcttc tgcggccaca cgtctggcaa ggtttccctg
     1201 agagacctcc gtacttttaa ggtggaacat gagtttgatg ccttctcagg aagtctgtca
     1261 gactttgatg tgcatggcaa cctgctagct gcctgtggct tctccagccg cctcactggc
     1321 ctggcctgcg accgtttcct caaggtgtat gatttgcgca tgatgcgtgc catcacacca
     1381 cttcaagtac atgtggatcc tgccttcttg cgcttcattc ctacatatac ttctcgtctt
     1441 gctatcatct ctcagtcagg gcagtgccaa ttctgtgaac ccacaggcct ggccaaccca
     1501 gccgatatct ttcatgtgaa tcctgtgggg cctctgctaa tgacatttga tgtgtcagcc
     1561 agcaagcagg ctctggcctt tggggattct gagggctgtg tgcacctctg gactgattcc
     1621 ccggagcctt ccttcaaccc ctactcccgt gagactgagt ttgctttgcc gtgtctcgtg
     1681 gactcactgc ctcctctgga ctggagccag gacctgctgc ctctttccct catccctgtc
     1741 ccactcacca ctgacacact tctctctgat tggcctgctg ccaactctgc tccagctccc
     1801 aggcgagcac cacccgtgga tgcagagatt ctgcgcacca tgaagaaggt gggcttcatt
     1861 ggctatgcgc ccaatccccg caccaggctg cgcaatcaga taccctacag actcaaggag
     1921 tcagacagtg aatttgacag cttcagccag gtcactgagt caccagtagg acgagaagag
     1981 gaaccacatc tccacatggt ttctaagaaa taccgcaagg tgaccatcaa atattccaag
     2041 ctagggctgg aggactttga cttcaaacac tacaataaga ccttgtttgc tggattagag
     2101 ccccacattc ccaacgccta ctgtaactgc atgatccagg tgctctattt cctggagcct
     2161 gtacgctgtc taattcaaaa ccacctttgc cagaaggagt tctgtctggc atgtgagctg
     2221 ggcttcctgt ttcacatgtt ggacctctct cgtggtgacc cttgccaggg caataatttt
     2281 cttcgggcat tccgtactat tcctgaggcc tcagccctcg gtctaatcct ggctgactca
     2341 gatgaggcct caggcaaggg caatctggcc aggctcattc agaggtggaa tcgcttcatt
     2401 ctcactcaac tgcatcaaga tatgcaggag ctggaaatac cacaggctta tcgaggtgct
     2461 ggaggcagca gcttttgctc atcgggggac tctgttattg ggcagctctt cagctgtgag
     2521 atggagaact gcagcctctg ccgctgtggc agtgagaccg tgcgagcctc atccactctg
     2581 cttttcacac tctcctaccc tgatgataaa actgggaaga actatgactt tgctcaggtg
     2641 ctgaagcgaa gcatctgcct ggaccagaat acacaggcct ggtgtgacac ctgtgaaaag
     2701 taccagccca cgattcagac ccgcaacatc cgccatctgc cagatattct tgtcatcaat
     2761 tgtgaggtga acagctcaaa agaggctgat ttctggagaa tgcaggctga ggttgccttc
     2821 aagatggcag taaagaaaca cggtggggaa atctccaaga acaaggaatt tgctttggct
     2881 gattggaagg aactagggag tccagagggt gtgctggtgt gtccctccat tgaggagttg
     2941 aagaacgtct ggcttccttt ctccattcgc atgaagatga ccaaaaacaa agggctggat
     3001 gtttgcaatt ggactgatgg ggatgagatg cagtggggcc cagccagggc agaggaggag
     3061 catggtgtct atgtgtatga cctgatggct actgtggtac acatcctgga ctcacgcaca
     3121 gggggcagcc tggtggctca catcaaagtt ggagagacct accaccagcg caaggagggc
     3181 gttactcacc agcagtggta tctgttcaat gactttctta ttgaacctat tgataagcat
     3241 gaagctgtgc agtttgacat gaattggaaa gtacctgcaa tcctttatta tgtcaaacgg
     3301 aatctcaatt ccagatacaa cctgaacatc aagaacccta ttgaggcaag tgtcttgctg
     3361 gctgaagcct cgctggcacg gaagcagcgg aaaacacata ctacctttat tccactgatg
     3421 ctgaatgaga tgccacagat tggggacctg gtgggtctgg atgctgagtt tgtcaccctt
     3481 aatgaggagg aagcagagtt acgcagtgat ggtaccaagt ctaccattaa accaagccag
     3541 atgtcagtag ccaggattac ctgtgttcgg ggccagggac ccaatgaggg tatccccttc
     3601 attgatgact acatctctac ccaggagcag gtggtggatt acttgactca atactcgggt
     3661 ataaagcctg gtgacctcga tgccaaaatt tcctccaagc acctaacaac tctcaagtct
     3721 acctacttaa agcttcgttt tctcattgac attggagtca agtttgtggg tcatggcctg
     3781 cagaaggact tccgggtcat caacctgatg gtgcccaagg accaagtcct tgacactgtc
     3841 tacctgttcc atatgccccg aaaacgaatg atttccctgc gattccttgc ttggtacttt
     3901 ctggacctga agattcaagg ggaaacccat gacagtattg aggatgcccg cacagccctt
     3961 cagctgtacc gaaagtatct ggagctaagc aaaaatggca ctgagcctga gtctttccac
     4021 aaggtgctca agggtcttta tgagaagggc agaaagatgg actggaaggt gcctgagcct
     4081 gagggccaaa caagtcccaa gaatgcagct gtcttctcct cagtgctggc gctctgccca
     4141 atttcttgta caaagtggtt cgataacgaa ttccgccccc cccccctaac gttactggcc
     4201 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4261 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4321 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4381 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4441 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4501 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     4561 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     4621 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     4681 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     4741 atggccacaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     4801 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     4861 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     4921 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     4981 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5041 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5101 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5161 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5221 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5281 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     5341 cccggtgcct gatgcatcga tgtcgacctg cagccaagct tatcgataaa ataaaagatt
     5401 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
     5461 gcttaagtaa cgccattttg caaggcatgg aaaatacata actgagaata gagaagttca
     5521 gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag
     5581 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca
     5641 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     5701 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     5761 ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg
     5821 cgtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     5881 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     5941 atgggtaaca gtttcttgaa gttggagaac aacattctga gggtaggagt cgaatattaa
     6001 gtaatcctga ctcaattagc cactgttttg aatccacata ctccaatact cctgaaatag
     6061 ttcattatgg acagcgcaga aagagctggg gagaattgtg aaattgttat ccgctcacaa
     6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8221 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8281 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     8341 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     8401 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     8461 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8521 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     8581 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     8641 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa
     8701 tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc
     8761 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     8821 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     8881 tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct
     8941 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
     9001 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     9061 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
     9121 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
     9181 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
     9241 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     9301 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     9361 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     9421 tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa
     9481 tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg
     9541 gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac caccgacccc
     9601 cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt
     9661 ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat
     9721 ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg
     9781 tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa
     9841 tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
     9901 tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg
     9961 ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta
    10021 g
//
