LOCUS       sequence-313927-        9676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP29
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      732..741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             741..764
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             777..803
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    819..843
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3580..3604)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3623..4122
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3658..3681)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4055..4074
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4143..4742
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4143..4162)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4639..4659
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    5328..5344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5352..5358,5359..5376,5377..5382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5397..5418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5535..5552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5706..6294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5786..5805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6465..7256,7257..7325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7088..7107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7326..7430)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7498..7516
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7554..7576)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7676..7695
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7889..7911
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8039..8058
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             8258..8774
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    8838..9179
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             9246..9662
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9592..9614
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9630..9646
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggggatc tgccaccatg gactacaagg atgacgatga caagctcgat ggaggatacc
      781 catacgatgt tccagattac gctgctcgag gttatcaaac aagtttgtac aaaaaagttg
      841 gcatgatatc tctaaaggta tgtggattca tccaaatttg gagccagaag actgggatga
      901 ctaagctgaa agaagctctc attgaaacag tgcaaagaca aaaggaaatt aaactggtgg
      961 tcactttcaa atctggaaaa tttataagaa tttttcagct gagcaacaac attagaagtg
     1021 tggtccttag acattgtaaa aaaagacaaa gtcacctgcg tttaactttg aaaaacaacg
     1081 tgttcttgtt tattgacaaa ttatcctaca gagatgctaa acagttgaat atgttcctgg
     1141 acataatcca ccaaaacaaa tctcagcaac ccatgaaatc tgatgatgat tggagtgtgt
     1201 ttgaaagcag gaatatgctg aaggaaattg acaaaacttc attttacagc atttgtaaca
     1261 agccaagtta tcagaagatg cctttgttta tgtcaaaatc accaacacat gtgaaaaagg
     1321 ggatattaga aaatcaaggt gggaaggggc aaaacacact atcatctgat gtacagacaa
     1381 atgaggacat tctgaaggaa gataaccctg taccaaacaa gaaatataag acagattcct
     1441 tgaaatatat acaaagcaat aggaagaacc catcaagttt agaggattta gaaaaagata
     1501 gagatttgaa actcgggcct tcattcaata ccaactgtaa tggaaatcct aacctagatg
     1561 agactgttct tgcaacccag actctcaatg ccaaaaatgg tttgacatct ccattggaac
     1621 cagagcacag ccagggtgac ccaagatgca acaaagccca ggtgcctctt gactctcatt
     1681 cacagcaact gcagcagggg ttccccaatt tgggaaacac ctgttacatg aatgcagttt
     1741 tacaatcgct atttgcaatt ccatcttttg ctgatgactt actcactcaa ggtgtcccat
     1801 gggaatatat tccctttgag gctcttatta tgaccttgac ccagctgctt gctttgaaag
     1861 atttctgtag tacaaagatc aagagagaat tacttgggaa tgttaaaaaa gtcatttcag
     1921 cagttgcaga aatattttct ggcaacatgc agaatgatgc tcatgagttt ttaggtcagt
     1981 gtttagacca gctgaaagaa gacatggaaa aattaaatgc cactttgaat actgggaaag
     2041 aatgtgggga tgaaaattca tctccacaaa tgcatgttgg tagtgctgcc accaaagtgt
     2101 ttgtttgccc tgttgttgct aattttgagt ttgaattgca gctctccctt atttgtaaag
     2161 cttgtggtca tgctgttctc aaggtagaac ctaataatta tctctccatc aacctgcacc
     2221 aagaaacaaa accacttcct ttgtccattc agaattcttt agatcttttc tttaaagaag
     2281 aagagcttga atataactgt cagatgtgta agcagaagag ttgtgttgca aggcatacat
     2341 ttagtaggct ctccagggtc cttatcattc atctgaaacg ctatagcttc aacaatgctt
     2401 ggttgctggt gaagaataac gagcaagttt atattcccaa atctttaagt ttatcttctt
     2461 attgcaatga aagcaccaaa ccacctcttc ccttgagcag tagtgcacct gttgggaaat
     2521 gtgaagtcct ggaagtctct caggagatga tttctgagat caacagccca ttgacaccat
     2581 caatgaagct gacctcagaa tccagtgatt ccctggttct acccgttgaa ccagacaaga
     2641 atgccgacct acaaagattc cagagagact gtggagatgc aagccaagag cagcatcaga
     2701 gagacctgga aaatggctct gcactagagt cagaattggt ccactttaga gatagggcaa
     2761 tcggtgaaaa ggagcttcca gtggctgact cactgatgga ccagggagac atttctcttc
     2821 ctgtgatgta tgaagatgga gggaagctga tcagcagccc agacacaagg cttgtcgagg
     2881 ttcatcttca agaggtgcct caacatccag aacttcagaa gtatgagaaa accaatacat
     2941 tcgtagagtt caattttgac agtgtcactg agtccaccaa tggcttttat gactgtaaag
     3001 aaaacaggat tccagaagga tctcaaggaa tggctgaaca gctccagcag tgtattgagg
     3061 agagcatcat agatgaattt cttcagcagg caccacctcc aggtgttagg aagctggatg
     3121 cccaggaaca tacagaagag accctcaatc agtctacaga attaagactt caaaaggctg
     3181 acctgaatca ccttggggca ctgggttctg acaacccagg aaacaaaaac attttagatg
     3241 cagagaacac aagaggtgaa gccaaggaac taacaagaaa cgtgaagatg ggggatcctc
     3301 tccaggccta cagactcatc agtgttgtca gccatatcgg gagctcccca aattcaggcc
     3361 attacatcag cgatgtgtat gactttcaga agcaggcctg gttcacatac aacgatctat
     3421 gtgtatcaga aatctcagag accaaaatgc aggaggcgag gcttcactct gggtatatct
     3481 tcttttacat gcacaatggg atttttgagg agctgttaag aaaagcagag aactctcggc
     3541 tacctagcac acaggcaggg gtgatccctc agggggaata accaactttc ttgtacaaag
     3601 tggttcgata acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     3661 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     3721 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     3781 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     3841 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     3901 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     3961 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     4021 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     4081 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     4141 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     4201 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     4261 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     4321 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     4381 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     4441 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     4501 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     4561 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     4621 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     4681 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     4741 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga
     4801 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     4861 ggtttggcaa gctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     4921 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     4981 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag
     5041 actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     5101 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     5161 tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata
     5221 ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa
     5281 atagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg ttatccgctc
     5341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7441 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7501 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     7561 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     7621 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     7681 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     7741 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     7801 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     7861 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa
     7921 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     7981 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     8041 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     8101 tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg
     8161 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     8221 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     8281 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
     8341 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
     8401 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     8461 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     8521 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     8581 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     8641 ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc
     8701 cgaatcgtgg actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc
     8761 gggggtcttt catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga
     8821 cccccccgcc gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc
     8881 gtgtttgtgc cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct
     8941 gtatctggcg gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga
     9001 gacgtcccag ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt
     9061 ggaatccgac cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc
     9121 cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc
     9181 agcgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa
     9241 attagggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     9301 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     9361 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     9421 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     9481 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct
     9541 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac
     9601 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg
     9661 ccggaattag atcctt
//
