LOCUS       sequence-313932-       10213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USPL1
FEATURES             Location/Qualifiers
     source          1..10213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      732..741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             741..764
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             777..803
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    819..843
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4117..4141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4160..4659
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4195..4218)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4592..4611
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4680..5279
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4680..4699)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5176..5196
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    5865..5881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5889..5895,5896..5913,5914..5919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5934..5955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6072..6089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6243..6831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6323..6342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7002..7793,7794..7862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7625..7644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7863..7967)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8035..8053
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8091..8113)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8213..8232
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8426..8448
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8576..8595
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             8795..9311
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9375..9716
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             9783..10199
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     10129..10151
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     10167..10183
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggggatc tgccaccatg gactacaagg atgacgatga caagctcgat ggaggatacc
      781 catacgatgt tccagattac gctgctcgag gttatcaaac aagtttgtac aaaaaagttg
      841 gcatggattc tccgaagatt ggaaatggtt tgccagtgat tggaccaggg actgatatag
      901 ggatatcttc actccacatg gtggggtatt tgggaaaaaa ttttgattca gctaaagttc
      961 catcagatga gtattgccct gcttgtagag agaagggaaa gttaaaagcc ttaaagactt
     1021 accgaattag ttttcaagaa tctatctttt tgtgtgagga tctgcagtgc atctatcctt
     1081 tgggctctaa atcacttaat aacctaattt ctcctgattt ggaagaatgt cacactccac
     1141 ataagcctca gaaaaggaag agcttagaaa gcagctataa ggattcactt cttttagcaa
     1201 attccaaaaa gactagaaat tatattgcta ttgacggtgg aaaagttttg aacagcaaac
     1261 ataatggaga agtatatgac gaaacctcgt caaacttacc tgatagtagt ggtcaacaga
     1321 atccaattag gacagctgat tccttggagc ggaatgagat tttggaagct gatactgttg
     1381 acatggctac tacaaaagat cctgctacag ttgatgtctc tggaactggc agaccttccc
     1441 ctcaaaatga aggatgtaca tctaaactgg aaatgccact ggagagcaaa tgtacatcat
     1501 ttccccaggc tttatgtgtc cagtggaaaa atgcttatgc tctctgttgg ttagactgta
     1561 tcctgtcagc tttggtgcac tcggaagagt taaagaacac cgtgactgga ctgtgctcga
     1621 aggaggaatc tatattctgg cggttgctta caaaatataa tcaagcaaat acacttctat
     1681 ataccagtca attgagtggt gttaaagatg gagattgtaa aaaacttacc tcagaaatat
     1741 ttgcagagat agagacctgt ctgaatgaag ttagagatga aatttttatt agccttcagc
     1801 cccagcttag atgcacatta ggtgatatgg aaagccctgt gtttgcattt cccctgctct
     1861 taaaactaga aacccacatt gaaaagctct tcctatattc tttttcttgg gactttgaat
     1921 gttcgcagtg tggacaccaa tatcaaaaca ggcatatgaa gagtctggtc acctttacaa
     1981 atgtcatccc tgagtggcac ccacttaatg ctgcccattt tggtccatgt aacaattgca
     2041 acagtaaatc acaaataaga aaaatggtat tagaaaaagt atctcccata ttcatgttgc
     2101 actttgtaga aggcttacca cagaatgact tgcagcacta tgcatttcat tttgaaggct
     2161 gtctttatca gataacttct gtaattcagt atcgagcaaa taatcatttt ataacatgga
     2221 ttttagatgc tgatggaagt tggctggaat gtgatgactt aaaaggccca tgttctgaaa
     2281 ggcacaagaa atttgaagtt cctgcttcag agatacatat tgttatttgg gaaagaaaaa
     2341 tatcccaagt gacagataaa gaagctgcct gccttccact taaaaagact aatgaccaac
     2401 acgctctcag taatgagaaa ccagtatctt taacatcgtg ttctgtgggt gatgctgcct
     2461 cagctgaaac agcctcagta actcacccta aagatatatc agttgcccct cgtactcttt
     2521 cacaggacac agctgtaact catggagatc atttactttc aggtccaaaa ggtttggttg
     2581 acaatatttt acctctgaca cttgaagaaa ctatccagaa aacagcctca gtttcacagt
     2641 taaattctga agctttcctg ttagaaaata aacctgtagc agaaaataca ggaattctca
     2701 aaaccaatac tttgctatca caagaatcac taatggcttc ttcagtatca gctccatgta
     2761 atgaaaagct tattcaagac caatttgtgg acataagttt tccatcccaa gttgtaaata
     2821 caaacatgca gtcagtacag ctgaatacag aagatactgt aaatactaaa tctgtgaata
     2881 atactgatgc tactggtctt atacagggag tgaagtcagt agaaattgag aaggacgctc
     2941 agttaaaaca attccttaca ccaaaaactg aacaattaaa accagaacgt gtcacatctc
     3001 aggtatctaa tttgaagaaa aaagaaacta cagcagattc tcaaaccaca acatctaagt
     3061 cattacagaa tcagtctctg aaagaaaatc agaagaagcc atttgtggga agttgggtta
     3121 aaggcttaat aagcaggggt gcttctttta tgccactctg tgtttcagct cataatagaa
     3181 acactataac tgatttacaa ccttcagtta aaggggtaaa taattttggt ggctttaaaa
     3241 ctaaaggtat aaaccagaag gccagccacg tatccaagaa agctcgtaag agtgcaagta
     3301 agcctcctcc catcagtaag ccaccagcag gccctccatc gtctaatggc acagctgccc
     3361 acccacatgc tcatgctgct tcagaagttt tggaaaagtc tggaagcacc tcatgtggag
     3421 ctcaactcaa ccacagttct tatgggaatg gtatttcttc agcaaaccat gaagacttgg
     3481 tggaaggtca gattcataaa cttcgtctaa aacttcgtaa aaagctaaag gcagaaaaga
     3541 agaaattagc tgctcttatg tcttccccgc aaagcagaac agttcgaagt gaaaatctag
     3601 aacaggtgcc ccaggatggg tctccaaatg attgtgaatc aatagaggac ttgttaaatg
     3661 agctaccata tccaattgat attgccagtg agtctgcatg caccactgtt cctggtgttt
     3721 ccctgtacag tagtcaaact catgaagaaa ttttagcgga attattgtct cctacacctg
     3781 tttcaacaga gctgtcagaa aatggggaag gtgactttag gtatttggga atgggagata
     3841 gtcatatccc accaccagta ccaagtgaat tcaatgatgt ttcccagaac acacatctga
     3901 gacaggacca taattattgt agccccacca agaaaaatcc atgtgaagtt cagccagact
     3961 ctctgacaaa taatgcctgc gttagaacat taaacttgga gagtccgatg aagactgata
     4021 ttttcgatga gtttttttcc tcctcagcat taaatgcttt agcaaatgac acattagacc
     4081 tacctcattt cgatgaatat ctgtttgaga attattgacc aactttcttg tacaaagtgg
     4141 ttcgataacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     4201 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     4261 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     4321 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     4381 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     4441 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     4501 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     4561 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     4621 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     4681 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     4741 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4801 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4861 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4921 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4981 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     5041 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     5101 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     5161 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     5221 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     5281 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa
     5341 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     5401 ttggcaagct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     5461 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     5521 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact
     5581 gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     5641 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     5701 catgggtaac agtttcttga agttggagaa caacattctg agggtaggag tcgaatatta
     5761 agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac tcctgaaata
     5821 gttcattatg gacagcgcag aaagagctgg ggagaattgt gaaattgtta tccgctcaca
     5881 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5941 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6001 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6061 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6121 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6181 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6241 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6301 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6361 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6421 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6481 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6541 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6601 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6661 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6721 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6781 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6841 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6901 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6961 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7021 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7081 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7141 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7201 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7261 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7321 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7381 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7441 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7501 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7561 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7621 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7681 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7741 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7801 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7861 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7921 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7981 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     8041 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     8101 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     8161 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     8221 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     8281 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8341 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8401 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga
     8461 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     8521 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     8581 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     8641 gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc
     8701 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat
     8761 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     8821 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga
     8881 gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct
     8941 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     9001 cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     9061 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     9121 tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg
     9181 atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt ttgcatccga
     9241 atcgtggact cgctgatcct tgggagggtc tcctcagatt gattgactgc ccacctcggg
     9301 ggtctttcat ttggaggttc caccgagatt tggagacccc tgcccaggga ccaccgaccc
     9361 ccccgccggg aggtaagctg gccagcggtc gtttcgtgtc tgtctctgtc tttgtgcgtg
     9421 tttgtgccgg catctaatgt ttgcgcctgc gtctgtacta gttagctaac tagctctgta
     9481 tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac
     9541 gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga
     9601 atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
     9661 ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc
     9721 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt
     9781 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
     9841 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
     9901 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     9961 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
    10021 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
    10081 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
    10141 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
    10201 gaattagatc ctt
//
