LOCUS       sequence-31395-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PLXNC1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             451..468
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(521..539)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      535..582
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(706..725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgcatc atcatcatca
       61 tcacagcagc ggcagagaaa acttgtattt ccagggcacc gtagccttaa atgtcgtttt
      121 tgaaaagatt ccggaaaatg agtcagctga tgtgtgtcgc aatatctcgg taaacgtctt
      181 agattgcgac actattgggc aggcgaaaga gaaaatcttc caggcttttc tgagcaaaaa
      241 cggtagtccg tatggcctgc agttaaacga aattggtctg gaattacaaa tggggacacg
      301 gcagaaggag ttattagata tcgactcgag tagcgttatc ctggaagatg gaatcacgaa
      361 gctgaataca atcggacact acgagattag caatgggtca actatcaaag tgttcaaaaa
      421 gtgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      481 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      541 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      601 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      901 ccacgttctt taatagtgga ctcttgttca
//
