LOCUS       sequence-313953-        9847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for mammalian overexpression of the siRNA resistant ER isoform of 
            rat USP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9847)
  TITLE     rat USP19 constructs
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-rUSP19ER-siRNAresistant
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(222..1013,1014..1082))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     845..864
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1083..1187)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1262..1281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1453..1832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1833..2036
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1987..2007
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2081..2100
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2081..2099
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2206..2215
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2218..2235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2236..2301
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(6654..6671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6660..6884
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6930..7358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7017..7036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7227..7248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7367..7387)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7372..7701
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7552..7687
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7768..8562
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7822..7841)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8432..8451
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8738..8859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8775..8794)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8829..8848
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8908..8924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8908..8924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8921..8943)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8932..8948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8956..8962,8963..8980,8981..8986))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9001..9022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9139..9156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9310..9847,1..51))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9390..9409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
       61 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      121 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      181 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      241 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
      301 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      361 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
      421 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
      481 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
      541 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
      601 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
      661 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
      721 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
      781 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
      841 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
      901 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
      961 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1021 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1081 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1141 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1201 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     1261 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     1321 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     1381 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     1441 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     1501 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     1561 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     1621 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1681 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1741 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1801 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     1861 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     1921 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     1981 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     2041 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     2101 agacccaagc tggctagtta agcttggtac cgagctcgga tccactagtc cagtgtggtg
     2161 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagccac catggcacat
     2221 catcatcatc atcacgacta caaagaccat gacggtgatt ataaagatca tgacatcgat
     2281 tacaaggatg acgatgacaa gcgcccgggc tgcaggaatt cgatgggtcc atgtgctggc
     2341 accagtgcta caggcccaag gagggggcct ccgggattgg aggaggcaac tagtaaaaaa
     2401 aaacaaaagg atcgagcaaa ccaggaaagt aaggatggag atcctaggag agtgtccatg
     2461 cctcgaaagg agcccaccaa agatgaactg ttgctcgatt ggagacagag ttcagataag
     2521 gtggttgtta agctgcgcgt gggaacaggt cccatatgtc tggaggaagt agatgctgct
     2581 ttcacagaca cggactgtgt ggtgaggctt ccagatggtc ggcagtgggg tggtgtcttc
     2641 tttgctgaaa tacaaagttc ttgtaccaaa gtgcagactc acaagggtgg tcttctacag
     2701 ttggcactac caaagaaggt gcctcttctc acgtggccct ctctcctgaa gaaacctcta
     2761 gggacccaag aactggtgcc aggattgcgg tgccaggaga acggacaaga actgtctccc
     2821 attgccctgg agccaggttc tgagccccgc agagctaaac aggaagctcg aaaccagaag
     2881 agggcccagg gccgtggtga ggtaggctca ggggctagcc ctggggcaca ggcagggccc
     2941 agtgccaaga gggctgttca tctctgcaga gggccagaag gtgaagggtc catggatggg
     3001 cctggccccc agggtgatgc cccatccttc ctgtccgact cagctacgca ggttgaggct
     3061 gaggagcagc tccatgttcc accagtgaac cctcaaacaa gtctcttggg ctcagagaag
     3121 aatttagccc ttttgacagt agagaagaca gtgtccccca ggagtgactc agtctcccca
     3181 gtcatgatcc ggaacagaga ccctgagaaa gatgaccatt tcaaagagga gatggcagtt
     3241 ggagctgatc ctgcagcctt agcggatgaa ccagagtcta tggtgaacct ggcatttgtc
     3301 aagaatgatt cgtatgagaa ggggccggat tcagtggtgg tgcacgtgta tgtgaaggag
     3361 agccgcaggg atacctctcg agtacttttc cgagagcagg acttcacgct gatcttccag
     3421 accagggatg gaaattttct gaggctgcat ccgggctgtg ggccccacac catcttccgc
     3481 tggcaggtga agctcaggaa cttgattgag ccagagcaat gtacgttttg tttcacggcc
     3541 tctcgaatcg atatctgcct ccggaagcgg cagagtcagc gctggggggg actggaggcc
     3601 cctgctacac gagtgggtgg tgcaaaggtt gccgtgccga caggtccaac ccctttggat
     3661 tcaacccctc caggaggtgg ccccctccct ctgactggcc aggaggaagc cagggctgta
     3721 gagaaggaaa aacccaaggc tcggtcagag gactcggggc tggatggtgt ggtggctcgc
     3781 acccccttgg agcatgttac cccaaagcca gaaccgcact tggcctcgcc caaacccaca
     3841 tgtatggtgc ctccaatgcc tcatagtcca gtgagtggag atagtgtgga ggaggatgaa
     3901 gaggaagaga agaaggtttg tctcccaggc tttactggcc tcgtcaactt agggaacacc
     3961 tgcttcatga atagtgtcat tcagtctctg tccaacactc gggagcttcg tgacttcttc
     4021 cacgaccgat cctttgaggc tgagattaac tacaataacc cattggggac tggtgggcgt
     4081 ctggctattg gcttcgctgt gctgctccgg gccctgtgga agggcactca ccaagccttt
     4141 caaccctcca agctaaaggc cattgtagca agcaaggcca gccagtttac aggctacgca
     4201 cagcatgatg cccaagagtt catggctttc ttgttggatg ggctgcatga agacctgaat
     4261 cggatccaaa acaaacccta cacagagact gtggactcag atgggcgacc tgacgaggtg
     4321 gtggctgaag aagcatggca gcggcacaag atgaggaacg actcattcat tgtggacctg
     4381 tttcagggcc agtacaagtc aaagctggtg tgccctgtgt gtgccaaggt ctccatcact
     4441 tttgacccgt tcctttatct gccggtaccc ttgccacaaa agcaaaaggt tctccccata
     4501 tattattttg ccagagagcc ccatagcaaa cccatcaagt tcctggtgag cgtcagcaag
     4561 gagaactcca gcgcaagcga agttttggag tccctctctc agagtgtcca cgtgaagcct
     4621 gagagcctgc gcctggctga ggtaattaag aatcgtttcc accgtgtttt cttgccctcc
     4681 cactcactgg atgctgtgtc gccaacggac gtgctcctgt gctttgagct gctatcccca
     4741 gaattggcta aggagagggt agtggtgcta gaggtgcagc agcgccccca ggtacccagc
     4801 atccctatct ccaagtgtgc agcctgccag cggaagcagc aatcagaaga tgaaaaactg
     4861 aagcgttgta cccgttgcta ccgtgtgggc tactgcaacc agttctgtca gaaaacccat
     4921 tggcctgacc acaaaggcct ctgccgcccc gagaacattg gctacccctt cctggtcagt
     4981 gtacctgctt cacgcctcac ttatgcccgt cttgctcagc tactagaagg ttatgcccgg
     5041 tactctgtga gcgtattcca accacccttc cagcctggcc gaatggcttt ggaatcacag
     5101 agccctggct gtaccacgtt gctttcaacc agctctctgg aggctgggga tagtgaaaga
     5161 gaacccattc agccttctga gctccagttg gtgactcctg tggctgaggg ggataccggg
     5221 gctcaccgca tgtggccacc tgctgatcgg ggtcctgtgc ctagcaccag tggaatttct
     5281 tccgagatgc tggccagtgg gcctatggaa gggtgttcct tgcttgctgg tgagagggtg
     5341 tctcggcctg aagctgctgt gcctgggtac cagcactcac gtgagtctgt gagcgcccac
     5401 acgccccagt tcttcatcta taaaattgat gcgtcaagcc gtgagcagcg gctggaggac
     5461 aaaggggaca ctccactgga gctaggtgat gactgtagcc tggctctggt gtggcggaac
     5521 aacgaacgcc ttcaggagtt tgtgctggtg gcctccaagg agctggagtg tgctgaagac
     5581 ccaggctccg ctggtgaggc tgcccgtgct ggccacttca ccctggacca gtgcctcaat
     5641 ctctttacac ggcctgaagt gctggcacct gaggaagcct ggtactgccc acagtgcaaa
     5701 cagcatcgag aggcctccaa gcagctgctg ctgtggcgcc taccaaatgt gctcatcgtg
     5761 cagctcaagc gtttctcctt tcgtagcttc atttggcgtg acaagatcaa tgacttggtg
     5821 gagtttccag ttcggaacct ggacttgagc aagttctgta ttggtcagaa agaggagcag
     5881 ctgcctagct atgacctgta tgcagtcatc aaccactacg gaggcatgat cggcggccac
     5941 tacactgcct gtgcacggct gcccagtgac cgtagtagcc agcgcagtga cgtgggctgg
     6001 cgcttgtttg atgacagcac ggtgacaaca gtagacgaga gccaggtggt gacacgctat
     6061 gcctatgtgc tcttctaccg tcggcggaac tctcctgtgg agaggccccc cagggcagct
     6121 cacgctgaac accacccaga cctaggccct gcagccgagg ctgctgccag ccaggcttcc
     6181 cggatttggc aggagctcga ggccgaggag gagatggtgc ccgaggggcc tgggcctctg
     6241 ggtccttggg ggccccaaga ctgggtgggg cccccgccac gtggccctac cacatcagac
     6301 gagggctgcc tccgatactt tgtcctgggt accgtggcgg ctttggtggc ccttgtgctc
     6361 aacgtattct atcctctggt atctcagagt cgctggagat gagctcccct gcaggcgcct
     6421 gctgtgagct ggcctaccta tgctcaccag gacaggcctg cctttgtggt gggagacagc
     6481 tctgggctct gagcctcagt gtatgtatct ggtgggagac ttgttgggac tacagcctct
     6541 gtgggggcag agtatcctag ggtgtgtgtc catccagttg tctgtcctcc tgtttctctg
     6601 acccttgtcc tagggcttgg ggaattcgat atcaagctaa acccgctgat cagcctcgac
     6661 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     6721 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     6781 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     6841 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     6901 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     7261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     7321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     7381 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7441 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7501 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7561 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7621 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     7681 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     7741 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     7801 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     7861 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     7921 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     7981 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     8041 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     8101 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     8161 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     8221 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     8281 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     8341 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     8401 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     8461 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     8521 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     8581 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8641 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8701 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8761 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8821 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     8881 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8941 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9001 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9061 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9121 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9841 tagcggt
//
