LOCUS       sequence-313955-        9512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for mammalian overexpression of the siRNA resistant CYT isoform of 
            rat USP19.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  TITLE     rat USP19 constructs
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-rUSP19CYT-siRNAresistant
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        147..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        527..730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     681..701
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     775..794
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        775..793
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      900..909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             912..929
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             930..995
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5013..5030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5019..5243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5289..5717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5376..5395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5586..5607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5726..5746)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5731..6060
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5911..6046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5973..5992
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6127..6921
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6181..6200)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6791..6810
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7097..7218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7134..7153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7188..7207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7267..7283)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7267..7283)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7280..7302)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7291..7307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7315..7321,7322..7339,7340..7345))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7360..7381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7498..7515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7669..8257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7749..7768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8428..9219,9220..9288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9051..9070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9289..9393)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9468..9487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
       61 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
      121 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
      181 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      361 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      421 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      541 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      601 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      661 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      721 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      781 gactcactat agggagaccc aagctggcta gttaagcttg gtaccgagct cggatccact
      841 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag
      901 ccaccatggc acatcatcat catcatcacg actacaaaga ccatgacggt gattataaag
      961 atcatgacat cgattacaag gatgacgatg acaagcgccc gggctgcagg aattcgatgg
     1021 gtccatgtgc tggcaccagt gctacaggcc caaggagggg gcctccggga ttggaggagg
     1081 caactagtaa aaaaaaacaa aaggatcgag caaaccagga aagtaaggat ggagatccta
     1141 ggagagtgtc catgcctcga aaggagccca ccaaagatga actgttgctc gattggagac
     1201 agagttcaga taaggtggtt gttaagctgc gcgtgggaac aggtcccata tgtctggagg
     1261 aagtagatgc tgctttcaca gacacggact gtgtggtgag gcttccagat ggtcggcagt
     1321 ggggtggtgt cttctttgct gaaatacaaa gttcttgtac caaagtgcag actcacaagg
     1381 gtggtcttct acagttggca ctaccaaaga aggtgcctct tctcacgtgg ccctctctcc
     1441 tgaagaaacc tctagggacc caagaactgg tgccaggatt gcggtgccag gagaacggac
     1501 aagaactgtc tcccattgcc ctggagccag gttctgagcc ccgcagagct aaacaggaag
     1561 ctcgaaacca gaagagggcc cagggccgtg gtgaggtagg ctcaggggct agccctgggg
     1621 cacaggcagg gcccagtgcc aagagggctg ttcatctctg cagagggcca gaaggtgaag
     1681 ggtccatgga tgggcctggc ccccagggtg atgccccatc cttcctgtcc gactcagcta
     1741 cgcaggttga ggctgaggag cagctccatg ttccaccagt gaaccctcaa acaagtctct
     1801 tgggctcaga gaagaattta gcccttttga cagtagagaa gacagtgtcc cccaggagtg
     1861 actcagtctc cccagtcatg atccggaaca gagaccctga gaaagatgac catttcaaag
     1921 aggagatggc agttggagct gatcctgcag ccttagcgga tgaaccagag tctatggtga
     1981 acctggcatt tgtcaagaat gattcgtatg agaaggggcc ggattcagtg gtggtgcacg
     2041 tgtatgtgaa ggagagccgc agggatacct ctcgagtact tttccgagag caggacttca
     2101 cgctgatctt ccagaccagg gatggaaatt ttctgaggct gcatccgggc tgtgggcccc
     2161 acaccatctt ccgctggcag gtgaagctca ggaacttgat tgagccagag caatgtacgt
     2221 tttgtttcac ggcctctcga atcgatatct gcctccggaa gcggcagagt cagcgctggg
     2281 ggggactgga ggcccctgct acacgagtgg gtggtgcaaa ggttgccgtg ccgacaggtc
     2341 caaccccttt ggattcaacc cctccaggag gtggccccct ccctctgact ggccaggagg
     2401 aagccagggc tgtagagaag gaaaaaccca aggctcggtc agaggactcg gggctggatg
     2461 gtgtggtggc tcgcaccccc ttggagcatg ttaccccaaa gccagaaccg cacttggcct
     2521 cgcccaaacc cacatgtatg gtgcctccaa tgcctcatag tccagtgagt ggagatagtg
     2581 tggaggagga tgaagaggaa gagaagaagg tttgtctccc aggctttact ggcctcgtca
     2641 acttagggaa cacctgcttc atgaatagtg tcattcagtc tctgtccaac actcgggagc
     2701 ttcgtgactt cttccacgac cgatcctttg aggctgagat taactacaat aacccattgg
     2761 ggactggtgg gcgtctggct attggcttcg ctgtgctgct ccgggccctg tggaagggca
     2821 ctcaccaagc ctttcaaccc tccaagctaa aggccattgt agcaagcaag gccagccagt
     2881 ttacaggcta cgcacagcat gatgcccaag agttcatggc tttcttgttg gatgggctgc
     2941 atgaagacct gaatcggatc caaaacaaac cctacacaga gactgtggac tcagatgggc
     3001 gacctgacga ggtggtggct gaagaagcat ggcagcggca caagatgagg aacgactcat
     3061 tcattgtgga cctgtttcag ggccagtaca agtcaaagct ggtgtgccct gtgtgtgcca
     3121 aggtctccat cacttttgac ccgttccttt atctgccggt acccttgcca caaaagcaaa
     3181 aggttctccc catatattat tttgccagag agccccatag caaacccatc aagttcctgg
     3241 tgagcgtcag caaggagaac tccagcgcaa gcgaagtttt ggagtccctc tctcagagtg
     3301 tccacgtgaa gcctgagagc ctgcgcctgg ctgaggtaat taagaatcgt ttccaccgtg
     3361 ttttcttgcc ctcccactca ctggatgctg tgtcgccaac ggacgtgctc ctgtgctttg
     3421 agctgctatc cccagaattg gctaaggaga gggtagtggt gctagaggtg cagcagcgcc
     3481 cccaggtacc cagcatccct atctccaagt gtgcagcctg ccagcggaag cagcaatcag
     3541 aagatgaaaa actgaagcgt tgtacccgtt gctaccgtgt gggctactgc aaccagttct
     3601 gtcagaaaac ccattggcct gaccacaaag gcctctgccg ccccgagaac attggctacc
     3661 ccttcctggt cagtgtacct gcttcacgcc tcacttatgc ccgtcttgct cagctactag
     3721 aaggttatgc ccggtactct gtgagcgtat tccaaccacc cttccagcct ggccgaatgg
     3781 ctttggaatc acagagccct ggctgtacca cgttgctttc aaccagctct ctggaggctg
     3841 gggatagtga aagagaaccc attcagcctt ctgagctcca gttggtgact cctgtggctg
     3901 agggggatac cggggctcac cgcatgtggc cacctgctga tcggggtcct gtgcctagca
     3961 ccagtggaat ttcttccgag atgctggcca gtgggcctat ggaagggtgt tccttgcttg
     4021 ctggtgagag ggtgtctcgg cctgaagctg ctgtgcctgg gtaccagcac tcacgtgagt
     4081 ctgtgagcgc ccacacgccc cagttcttca tctataaaat tgatgcgtca agccgtgagc
     4141 agcggctgga ggacaaaggg gacactccac tggagctagg tgatgactgt agcctggctc
     4201 tggtgtggcg gaacaacgaa cgccttcagg agtttgtgct ggtggcctcc aaggagctgg
     4261 agtgtgctga agacccaggc tccgctggtg aggctgcccg tgctggccac ttcaccctgg
     4321 accagtgcct caatctcttt acacggcctg aagtgctggc acctgaggaa gcctggtact
     4381 gcccacagtg caaacagcat cgagaggcct ccaagcagct gctgctgtgg cgcctaccaa
     4441 atgtgctcat cgtgcagctc aagcgtttct cctttcgtag cttcatttgg cgtgacaaga
     4501 tcaatgactt ggtggagttt ccagttcgga acctggactt gagcaagttc tgtattggtc
     4561 agaaagagga gcagctgcct agctatgacc tgtatgcagt catcaaccac tacggaggca
     4621 tgatcggcgg ccactacact gcctgtgcac ggctgcccag tgaccgtagt agccagcgca
     4681 gtgacgtggg ctggcgcttg tttgatgaca gcacggtgac aacagtagac gagagccagg
     4741 tggtgacacg ctatgcctat gtgctcttct accgtcggcg gaactctcct gtggagaggc
     4801 cccccagggc agctcacgct gaacaccacc cagacctagg ccctgcagcc gaggctgctg
     4861 ccagccaggg actaggccct ggccaggccc ccgaggtggc ccccacgcgg acagcccctg
     4921 aacgattcgt cccccctgtg gaccgcccag cccccacgta cagcaacatg gaggaggtcg
     4981 attagcaggt ccgtcgaaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5041 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5101 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5161 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5221 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     5281 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5341 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5401 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5461 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5521 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5581 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5641 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5701 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5761 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5821 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5881 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5941 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6001 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6061 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     6121 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6181 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6241 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6301 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6361 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6421 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6481 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6541 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6601 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     6661 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6721 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6781 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6841 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6901 cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg accaagcgac
     6961 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7021 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7081 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7141 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7201 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7261 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7321 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7381 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7441 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7501 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7561 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8101 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9421 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     9481 gatgccgcat agttaagcca gtatctgctc cc
//
