LOCUS       sequence-31397-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SGF29 (3LX7)
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(507..518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             593..610
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(663..681)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      677..724
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(848..867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcctctgaa tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcggtga caagccccca cccctctgtg
      121 gggccatccc tgcctcagga gactacgtgg ccagacctgg agacaaggtg gctgcccggg
      181 tgaaggccgt ggatggggac gagcagtgga tcctggccga ggtggtcagt tacagccatg
      241 ccaccaacaa gtatgaggta gatgacatcg atgaagaagg caaagagaga cacaccctga
      301 gccggcgccg tgtcatcccg ctgccccagt ggaaggccaa cccggagacg gaccctgagg
      361 ccttgttcca gaaggagcag ctcgtgctgg ccctgtatcc ccagactacc tgcttctacc
      421 gcgccctgat ccatgcgccc ccacagcggc cccaggatga ctactcggtc ctgtttgaag
      481 acacctccta tgcagatggc tattcccctc ccctcaatgt ggctcagaga tacgtggtgg
      541 cttgtaagga acccaagaaa aagtgatgac gaagcttgcg gccgcactcg agcaccacca
      601 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      661 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      721 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      781 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      841 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ct
//
