LOCUS       sequence-313979-        2213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0437
FEATURES             Location/Qualifiers
     source          1..2213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..46
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     280..297
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(321..378)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      567..610
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(614..632)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             794..1453
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(860..879)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1474..1568
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(1751..2213,1..126)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
       61 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagcctg
      121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      181 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      241 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      301 gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa aagggcaggg tggtgacacc
      361 ttgccctttt ttgccggact gcagggtctc gagtatatat acaaatcagt cactactgct
      421 ttcaatgaat agcaaaacct tatatagaca caagtggctg agctggtgat caacacatgc
      481 tggtgtgcat aattgatttt tgtttttgac ttttcatcaa tcttcctcac taaaacgaca
      541 cgcctccctc ctgagaccga attccagaaa tcatccttag cgaaagctaa ggattttttt
      601 tatctgaaat tctgcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      661 gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac gtaagaggtt ccaactttca
      721 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
      781 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
      841 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
      901 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
      961 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc
     1021 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     1081 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     1141 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     1201 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     1261 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     1321 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     1381 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     1441 gggcggggcg taatttgata tcgagctcgc ttggactcct gttgatagat ccagtaatga
     1501 cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg
     1561 gtgagaatcc aagcctcgag ctgtcagacc aagtttactc atatatactt tagattgatt
     1621 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1681 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1861 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga aca
//
